养殖半滑舌鳎肝胰、中肾、鳃、头肾、脾和心中酸性磷酸酶、碱性磷酸酶和过氧化物酶的组织化学定位  

Histochemical localization of acid phosphatase,alkaline phosphatase and peroxidase in the hepatopancreas,mid-kidney,gill,head kidney,spleen and heart of cultured Cynoglossus semilaevis Günther

作　　者：王一泽[1] 孙敬锋[1] 刘军锋 郭永军[1] 邢克智[1] 吕爱军[1] Yeong Yiksung Wang Yize;Sun Jingfeng;Liu Junfeng;Guo Yongjun;Xing Kezhi;Lv Aijun(Tianjin Key Lab of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, China;Tianjin Yushengtang Biotechnology Co.,Ltd., Tianjin 300404, China)

机构地区：[1]天津农学院水产学院天津市水产生态及养殖重点实验室,天津300384 [2]天津市渔生堂生物技术有限公司,天津300404

出　　处：《中国组织化学与细胞化学杂志》2017年第3期229-234,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：天津市应用基础与前沿技术研究计划项目(15JCZDJC34000;16JCZDJC33500);国家自然科学基金项目(31272692)

摘　　要：目的研究半滑舌鳎肝胰、中肾、鳃、头肾、脾和心中酸性磷酸酶(ACP)、碱性磷酸酶(ALP)和过氧化物酶(POX)的分布及组织定位。方法取健康半滑舌鳎肝胰、中肾、鳃、头肾、脾和心组织进行固定,冰冻切片后进行酶组织化学染色和光密度定量统计分析。结果 ACP活性部位为棕色,主要分布于肝胰小叶间胆管和静脉,中肾肾小体中的肾小球和肾间质的肾小管,头肾和脾中的巨噬细胞以及心肌层中,在鳃中未见有分布;ALP活性部位被染为蓝紫色,主要分布于肝胰的胰腺腺泡内,中肾肾间质的肾小管,鳃的鳃丝血管和上皮细胞,脾静脉的管壁处和椭圆体,心外膜和肌膜上以及头肾的血窦腔内皮;POX活性部位被染为茶褐色,主要分布于肝胰小静脉和肝血窦内的血细胞,中肾肾间质的血细胞,鳃的鳃丝血管、鳃小片血窦和上皮细胞,头肾、脾内的血细胞以及心的心肌层和血细胞中。ACP活性由大到小依次为心、肝胰、脾、中肾、头肾;ALP活性由大到小依次为中肾、鳃、头肾、脾、心、肝胰;POX活性由大到小依次为脾、头肾、肝胰、中肾、鳃、心。结论 ACP、ALP和POX在半滑舌鳎6种组织中分布特点不同,其活性大小在不同组织中有显著差异。Objecive To investigate the distribution and histochemical localization of acid phosphatase(ACP),alkaline phosphatase(ALP)and peroxidase(POD)in the hepatopancreas,mid-kidney,gill,head kidney,spleen and heart of Cynoglossus semilaevis Günther.Methods Tissue samples were taken from the hepatopancreas,mid-kidney,gill,head kidney,spleen and heart of healthy Cynoglossus semilaevis Günther,fixed and then frozen sectioned.Enzyme histochemical staining was performed on the sections,and the mean optical density(MOD)was measured for quantitative analysis.Results ACP stain was brown,mainly located in the interlobular bile duct and vein of hepatopancreas,glomerulus in renal corpuscle and tubule in interstitium of the mid-kidney,macrophages in head kidney,spleen and myocardium,while absent in the gill.The blue-purple-stained ALP was detected in the pancreatic acinus of hepatopancreas,tubule in the interstitium of mid-kidney,blood vessel and epithelial cells of gill filaments,venous wall and the ellipsoid of spleen,the adventitia and sarolemma of heart,and the blood sinus of head kidney.The dark-brown-stained POX was localized in the blood cells in the vein and hepatic sinusoid of hepatopancreas and those in the interstitium of mid-kidney,blood vessel of gill filaments,sinusoid and epithelial cells of gill lamellae,blood cells of head kidney,spleen and heart,and myocardium.ACP activity was in descending order in the heart,hepatopancreas,spleen,mid-kidney and head kidney;ALP activity was in descending order in the mid-kidney,gill,head kidney,spleen,heart and hepatopancreas;POX activity was in descending order in the spleen,head kidney,hepatopancreas,mid-kidney,gill and heart.Conclusion The distribution of ACP,ALP,and POX vary in the six types of tissues investigated,and there are significant differences in their expressions and activities.

关 键 词：半滑舌鳎 酶组织化学 酸性磷酸酶 碱性磷酸酶 过氧化物酶 

分 类 号：Q174[生物学—水生生物学]