猪萨佩罗病毒间接免疫荧光方法的建立与初步应用  

Establishing the indirect immunofluorescence assay for antibody detection from Porcine sapelovirus

作　　者：彭旺[1] 唐小明[2] 葛猛[1] 杨涛涛[1] 屈泰龙[1] 余兴龙[1] PENG Wang;TANG Xiao ming;GE Meng;YANG Taotao;QU Tailong;YU Xinglong(College of Veterinary Medicine, Hunan Agriculture University, Changsha 410128, China;Animal Disease Control Center of Hunan Province, Changsha 410007, China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]湖南省动物疫病预防控制中心,湖南长沙410007

出　　处：《湖南农业大学学报（自然科学版）》2017年第4期423-426,共4页Journal of Hunan Agricultural University(Natural Sciences)

摘　　要：以分离的猪萨佩罗病毒PSV Hu N1/2016株感染PK15细胞,制备抗原板,建立检测猪血清中PSV抗体的间接免疫荧光(IFA)方法。结果表明:一抗最佳稀释浓度为1∶300;FITC标记的羊抗猪荧光二抗最佳稀释浓度为1∶300。该方法特异性强,敏感度高,既能将PSV抗体与PRRSV、FMDV、PCV、CSFV抗体区分开,也可以检测到中和效价0.128的阳性血清。用该方法检测湖南省部分规模化猪场208份临床血清样品的总阳性率为68%,表明PSV在湖南省流行普遍,且其感染主要发生在保育阶段。Indirect immunofluorescence assay(IFA)for detecting antibody of Porcine sapelovirus(PSV)from porcineserum was established by employed the strain PSV HuN1cultured in PK–15cells as antigen coating in plate.Theprimary antibody and fluorescent secondary antibody in the test were diluted to300times.The results showed that theapproach not only had high specificity and sensitivity which could discriminate PSV from other porcine virus antigens(such as PRRSV,FMDV,PCV,and CSFV),but also could detect positive serum sample with concentration low to0.128SN titer.The fact detected by the established method that68%samples out of208serums from commercial farmsscattered in Hunan province were positive indicated that the PSV was widely prevalent in the region,and the infectionwas mainly occurred in the weaning period.

关 键 词：猪萨佩罗病毒 间接免疫荧光 抗体检测 湖南 

分 类 号：S858.28[农业科学—临床兽医学]