西瓜四倍体与二倍体正反交果实发育过程中内源激素的变化  

The Changes of Endogenous Hormones in the Cross and Reciprocal Cross of Watermelon Tetraploid and Diploid

作　　者：李涵[1,2] 王志伟[1,3] 孙波[1] 邹甜[1] 孙小武[1] WANG Zhi-wei;SUN Bo;LI Han;ZOU Tian;SUN Xiao-wu(College of Horticulture, Hunan Agricultural University, Changsha 410128, PRC;Hunan Vegetable Research Institute, Changsha 410125,PRC;Hunan Crop Research Institute, Changsha 410125, PRC)

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]湖南省作物研究所,湖南长沙410125 [3]湖南省蔬菜研究所,湖南长沙410125

出　　处：《湖南农业科学》2017年第6期1-3,共3页Hunan Agricultural Sciences

基　　金：湖南省研究生创新培养项目(CX2014B303);西甜瓜产业技术体系(CARS-26-09)

摘　　要：为了探究西瓜四倍体与二倍体反交种子败育的原因,以蜜枚的二倍体和四倍体为材料,采用酶联免疫法(ELISA)对授粉后1~13 d果实内赤霉素(GA)、玉米素核苷(ZR)、脱落酸(ABA)和生长素(IAA)4种内源激素进行测定。结果表明:蜜枚二倍体与四倍体西瓜正交果实发育过程中,ZR和ABA呈现升高-降低-升高-降低的趋势,GA_3和IAA呈现降低-升高-降低-升高的趋势,蜜枚二倍体与四倍体西瓜反交授粉后3~4 d ABA含量较高,授粉后7 d GA和IAA含量较低,授粉后5 d GA_3平均水平低于正交。这样的差异可能就是反交种子败育的原因。To explore the reasons of seed abortion of tetraploid and diploid crosses of watermelon,,genotypes with“MiMei”of diploidand tetraploid as material,using enzyme-linked immunoassay(ELISA)for1-13d after pollination fruit in gibberellin(GA),zeatinnucleoside(ZR)and abscisic acid(ABA)and auxin(IAA),four kinds of endogenous hormones were determined.The results showed thatthe ZR and ABA increased-up-lower-to reduce the trend,the GA3and IAA showed lower-higher-lower-rising trend,in the developmentof the orthogonal fruits of diploid and tetraploid watermelon.The content of ABA were high in3-4d after cross-pollination of diploid andtetraploid of“MiMei”watermelon.After pollination,the content of GA and IAA was lower in7d,and the content of GA3averaged lowerthan that of orthogonal in5d after pollination.Such a difference may be the reason for the reverse seed failure.

关 键 词：西瓜 四倍体 二倍体 正交 反交 内源激素 

分 类 号：S651[农业科学—果树学]