酵母培养物对凡纳滨对虾肠道菌群结构的影响  

Effect of Yeast Culture on Intestinal Microbiota of Litopenaeus vannamei

作　　者：何远法 迟淑艳[1] 谭北平[1,2] 张含乐 董晓慧[1,2] 杨奇慧[1] 刘泓宇[1] 章双[1] HE Yuan-fa;CHI Shu-yan;TAN Bei-ping;ZHANG Han-le;DONG Xiao-hui;YANG Qi-hui;LIU Hong-yu;ZHANG Shuang(Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;South China Sea Bio-Resource Exploition and Utilization Collaborative Innovation Center, Guangzhou 510275, China;Beijing Enhalor Biotechnology Co., Ltd, Beijing 100081, China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]南海生物资源开发与利用协同创新中心,广东广州510275 [3]北京英惠尔生物技术有限公司,北京100081

出　　处：《广东海洋大学学报》2017年第4期21-27,共7页Journal of Guangdong Ocean University

基　　金：农业部公益性行业科研专项(201003020);广东省教育厅高等学校高层次人才项目(粤财教[2013]246)

摘　　要：在基础饲料中分别添加质量分数0(对照)和0.30%的酵母培养物,配制2种等氮等脂饲料(分别记为Y0、Y0.3组),投喂初始体质量为(1.20±0.01)g的凡纳滨对虾(Litopenaeus vannamei)56 d。提取凡纳滨对虾肠道总DNA,采用Illumina Mi Seq高通量测序比较样品菌群组成和多样性,研究饲料中添加酵母培养物对凡纳滨对虾肠道菌群结构的影响。结果表明,Y0.3组对虾肠道分类单元(OTUs)数量、丰富度指数(Chao1、ACE值)、多样性指数(Shannon、np Shannon指数)与Y0组差异无统计学意义(P>0.05);从物种分类树发现,两组的主要细菌类群均为变形菌门(Proteobacteria),其中,γ-变形菌纲是对虾肠道的最主要菌群,所占比例达69.48%;从科分类水平看,Y0.3组对虾肠道菌群链球菌科(Streptococcaceae)比Y0组提高4.98%;从优势属水平来看,Y0.3组对虾肠道的乳球菌属(Lactococcus)高于Y0组,差异有统计学意义(P<0.01);Y0.3组对虾肠道的发光杆菌属(Photobacterium)低于Y0组,差异有统计学意义(P<0.05)。饲料中添加质量分数0.30%酵母培养物可改善凡纳滨对虾肠道菌群多样性。A yeast culture was added to the base diet at mass fraction0(control group)and0.30%,andtwo isonitrogenous and isolipid diets,named Y0and Y0.3respectively,were prepared.Litopenaeusvannamei with an average initial weight of1.20±0.01g were fed with the two diets for56days.Afterthe test,the intestine from Litopenaeus vannamei was extracted in order to acquire the total DNA.Illumina MiSeq high-throughput sequencing was used to compare composition and the diversity ofintestinal flora.Results showed that,though the operational taxonomic units(OTUs)number,Chao,ACE,Shannon and npShannon indexs of Y0.3group obtained higher average value than that of the Y0group,there were no significant difference between Y0.3group and those fed with Y0group(P>0.05).With respect to the phylogenetic tree of species classification,the main bacteria phyla,Proteobacteria,found in the intestine of shrimp fed with Y0.3was similar to the shrimp fed with Y0.The proportion ofclassγ-Proteobacteria in the intestinal microbiota reached69.48%.From the dominant family level ofbacterial community,the number of Streptococcaceae in Y0.3group were higher than those in Y0groupwhich had4.98%.From the dominant genus level of bacterial community,the proportion ofLactococcus in Y0.3group were significantly higher than those in Y0group(P<0.01).The proportionof Photobacterium in Y0.3group were also significantly lower than those in Y0group(P<0.05).It canbe concluded that the addition of dietary0.30%yeast culture improved diversity of intestinal microbiotafor Litopenaeus vannamei.

关 键 词：酵母培养物 凡纳滨对虾 肠道菌群 菌群结构 多样性 高通量测序 

分 类 号：S963.73[农业科学—水产养殖] S966.129[农业科学—水产科学]