百事乐胶囊对慢性应激抑郁大鼠海马磷脂酰肌醇-3激酶信号通路相关因子的影响  

Effects of Baishile Capsules on Hippocampal PI3K Signaling Pathway in Chronic Mild Unpredictable Stress Rats

作　　者：孟盼 杜青 张秀丽 柳卓 向韵 刘林[2] 王宇红[1,2] MENG Pan;DU Qing;ZHANG Xiu-li;LIU Zhuo;XIANG Yun;LIU Lin;WANG Yu-hong(Training Bases of Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha 410208, China;First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China)

机构地区：[1]湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医药信息杂志》2017年第8期41-45,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81403387);湖南省教育厅一般项目(14C0862);中医内科学省部共建教育部重点实验室开放基金(ZYNK201503);湖南中医药大学校级青年基金(99820001-147)

摘　　要：目的观察百事乐胶囊对慢性轻度不可预见性应激(CUMS)大鼠海马磷脂酰肌醇-3激酶(PI3K)信号通路关键因子表达的影响,探讨其抗抑郁的作用机制。方法将SD大鼠随机分为空白组、模型组、氟西汀组和百事乐胶囊高、中、低剂量组,每组10只。采用CUMS加孤养的方式建立抑郁模型,造模同时灌胃给药,连续21 d。采用新奇摄食实验和Open-field实验评价行为学变化,ELISA检测血清单胺神经递质,免疫荧光和Western blot检测大鼠海马PI3K、蛋白激酶B(AKT)、血清糖皮质激素调节蛋白激酶1(SGK1)表达。结果与空白组比较,模型组大鼠摄食潜伏期显著延长,水平和垂直活动次数明显减少(P<0.01),血清单胺神经递质含量下降(P<0.01),海马PI3K、AKT表达显著降低且SGK1表达显著升高(P<0.05,P<0.01);与模型组比较,百事乐胶囊高、中剂量组大鼠摄食潜伏期显著缩短(P<0.05),水平和垂直活动次数显著增加(P<0.01),血清单胺神经递质含量显著升高(P<0.05,P<0.01),海马PI3K、AKT显著增加且SGK1表达显著降低(P<0.05,P<0.01)。结论百事乐胶囊可通过缓解模型大鼠抑郁样行为,调控海马PI3K信号通路关键因子,发挥其抗抑郁的功效。Objective To observe the effects of Baishile Capsules on the hippocampal PI3K signaling pathway in chronic mild unpredictable stress(CUMS)rats;To discuss its mechanism of action for anti-depression.Methods SD rats were randomly divided into6groups,control group,model group,fluoxetine group,Baishile Capsules high-,medium-and low-dose groups,with10rats in each group.The cums depression model was established,and gavage for medication was conducted at the same time for21days in a row.The behavioral changes of rats in each group were detected by the novel feeding experiment and Open-field experiment;monoamine neurotransmitter in serum were detected by ELISA;the expressions of PI3K,AKT and SGK1in rat hippocampus were detected by immunofluorescence and Western blot test.Results Compared with the control group,feeding latency of rats in model group was significantly prolonged(P<0.01),horizontal and vertical activities were significantly reduced(P<0.01);the content of serum monoamine neurotransmitter decreased(P<0.01);the expressions of PI3K and AKT in hippocampus decreased,while the expression of SGK1increased(P<0.01,P<0.05).Compared with the model group,Baishile Capsule high-and medium-dose groups can significantly shorten the incubation period of feeding rats(P<0.05),and increase the number of horizontal and vertical activities(P<0.01);the5-HT and NE levels in serum were significantly elevated(P<0.05,P<0.01);the expressions of PI3K,AKT in hippocampal increased;the expression of SGK1decreased(P<0.05,P<0.01).Conclusion Baishile Capsules can alleviate the depression like behavior in rat models,and regulate key factors of hippocampal PI3K signaling pathway,so as to exert antidepressant effects.

关 键 词：百事乐胶囊 抑郁症 海马 磷脂酰肌醇-3激酶 蛋白激酶B 血清糖皮质激素调节蛋白激酶1 单胺神经递质 大鼠 

分 类 号：R285.5[医药卫生—中药学]