不同干燥条件连翘叶茶有效成分含量比较  

Comparison of Effects of Different Drying Methods on Contents of Effective Components of Forsythiae Fructus Leaves Tea

作　　者：麻景梅[1,2] 王迎春[1,2] 李琛[1,2] 王梦[1,2] 郑倩[3] 牛丽颖[1,2] MA Jing-mei;WANG Ying-chun;LI Chen;WANG Meng;ZHENG Qian;NIU Li-ying(Hebei University of Chinese Medicine, Shijiazhuang 050091, China;Hebei TCM Formula Granule Engineering&Technology Research Center, Shijiazhuang 050091, China;Shijiazhuang Traditional Chinese Medicine Hospital, Shijiazhuang 050001, China)

机构地区：[1]河北中医学院,河北石家庄050091 [2]河北省中药配方颗粒工程技术研究中心,河北石家庄050091 [3]石家庄市中医院,河北石家庄050001

出　　处：《中国中医药信息杂志》2017年第8期76-79,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河北省食品药品监督管理局普通项目(PT2014056);河北省高等学校科学技术研究重点项目(ZD2015001)

摘　　要：目的比较不同干燥条件下连翘叶茶中总黄酮、连翘酯苷A和连翘苷含量,确定连翘叶茶最佳干燥方式。方法采用紫外分光光度法,以亚硝酸钠-三氯化铝-氢氧化钠溶液体系为显色剂,在波长500 nm处测定总黄酮含量;采用HPLC同时测定连翘酯苷A和连翘苷含量,色谱柱为Diamosil C18(2)柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%乙酸水溶液,梯度洗脱,检测波长277 nm,柱温30℃,流速1.0 m L/min。结果不同干燥条件连翘叶茶中总黄酮含量基本相同;不同干燥条件连翘叶茶中连翘酯苷A和连翘苷含量顺序为:通风阴干干燥>真空干燥>鼓风干燥。结论不同干燥条件对连翘叶茶中总黄酮含量无明显影响,对连翘酯苷A和连翘苷的含量影响较大,通风阴干比鼓风干燥和真空干燥更有利于连翘酯苷A和连翘苷成分的保存。Objective To compare the contents of total flavonoids,forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions;To determine the best drying method for Forsythiae Fructus leaves tea.Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent,total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of500nm.HPLC was used to determine the contents of forsythiaside A and phillyrin.The analysis was performed on a Diamosil C18(2)column(4.6mm×250mm,5μm);the mobile phase was composed of acetonitrile and0.4%acetic acid with gradient elution;the detection wavelength was set at277nm;the flow rate was1.0mL/min at column temperature of30℃.Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same;the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was:ventilated drying>vacuum drying>blast drying.Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea,but have obvious effects on the contents of forsythiaside A and phillyrin.Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.

关 键 词：连翘叶茶 总黄酮 连翘酯苷A 连翘苷 紫外分光光度法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]