基于HPLC指纹图谱对救必应与其伪品的鉴别研究  

Identification of Ilicis Rotundae Cortex and Its Adulterant Based on HPLC Fingerprint

作　　者：马玉翠[1] 王淳[1] 王蔚[2] 吴翠[1] 杜宁[2] 孙文[1] 李慧[1] 巢志茂[1] Ma Yu-cui;WANG Chun;WANG Wei;WU Cui;DU Ning;SUN Wen;LI Hui;CHAO Zhi-mao(Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Beijing Center for Physical and Chemical Analysis, Beijing 100094, China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京市理化分析测试中心,北京100094

出　　处：《中国中医药信息杂志》2017年第8期89-92,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家质量监督检验检疫总局行业科研专项(201210209)

摘　　要：目的建立救必应与其伪品米碎木的HPLC指纹图谱,进行真伪鉴别。方法收集各地名为"救必应"的药材,采用HPLC指纹图谱方法对救必应及其伪品米碎木进行检测,采用《中药色谱指纹图谱相似度评价系统2004A版》对指纹图谱进行评价。结果建立了救必应和米碎木的HPLC指纹图谱,方法学考察符合指纹图谱技术要求。救必应与其伪品米碎木指纹图谱轮廓特征明显不同,救必应有31个共有峰,米碎木有28个共有峰。主成分分析可将样品分为2组,聚类分析也可将样品分为2类。结论采用HPLC指纹图谱及其数据分析,可以对救必应与其伪品进行鉴别,为救必应的质量评价提供有效方法。Objective To establish HPLC fingerprints for the identification of Ilicis Rotundae Cortex(Jiubiying),dried bark of Ilex rotunda Thunb.,and its adulterant,dried bark of Ilex godajam(Colebr.)Wall..Methods Some samples named“Jiubiying”were collected from some medicine markets and drugstores.The HPLC fingerprints were obtained to detect Jiubiying and Ilex godajam.The software“Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine(2004A edition)”was used to evaluate fingerprints.Results The fingerprints of Jiubiying and Ilex godajam were established.Methodological study met the technical requirements of fingerprints.There were31and28common peaks in Jiubiying and Ilex godajam,respectively.Jiubiying and Ilex godajam could be classified into two clusters by principal component analysis and hierarchical clustering analysis.Conclusion Jiubiying and Ilex godajam can be identified by HPLC fingerprints.This method can provide an effective method for quality evaluation of Jiubiying.

关 键 词：救必应 米碎木 高效液相色谱法 指纹图谱 主成分分析 质量控制 

分 类 号：R284.1[医药卫生—中药学]