外源施加AsA和MeJA对乙烯利诱导水稻叶片衰老的影响  

Effects of Exogenous Ascorbic Acid and Methy Jasmonate on Ethephon-induced Leaf Senescence in Rice

作　　者：魏燕霞[1] 陆俐娜 彭长连[1] WEI Yan-xia;LU Li-na;PENG Chang-lian(Guangdong Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China)

机构地区：[1]广东省植物发育生物工程重点实验室华南师范大学生命科学学院,广州510631

出　　处：《热带亚热带植物学报》2017年第4期348-356,共9页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(31270287);广东省自然科学基金重点项目(2015A030311023)资助~~

摘　　要：以野生型水稻(Oryza sativa)株系中花11(ZH-11)及其抗坏血酸合成关键酶基因GLDH下调株系(GI-2)为材料,研究了外源抗坏血酸(AsA)与茉莉酸甲酯(MeJA)对乙烯利诱导下水稻叶片早衰现象的影响。结果表明,外源AsA提高了水稻GI-2中的抗坏血酸含量、Rubisco含量及叶绿素的含量,减缓了其光合特性参数的下降速率,但对水稻ZH-11没有显著影响。外源MeJA降低了两株系的抗坏血酸、Rubisco及叶绿素含量,加快了叶内光合特性参数的下降速率,且对ZH-11的影响大于GI-2。因此,外源AsA处理能有效缓解乙烯利诱导的水稻叶片早衰现象,使叶片的衰老进程得以延缓,而外源MeJA作用相反。The effects of exogenous ascorbic acid(AsA)and methy jasmonate(MeJA)on rice(Oryza sativa)leaf senescence induced by ethephon was investigated using wild type of cultivar line Zhonghua11(ZH-11)and the mutant line GI-2with down-regulated GLDH gene,which GLDH was a key enzyme in AsA biosynthesis path.The results showed that exogenous AsA increased ascorbic acid content in GI-2.Likewise,the contents of Rubisco protein and chlorophyll contents increased significantly after treated by AsA,and the descending rate of photosynthetic parameters decreased,while AsA treatment had no significant effects on ZH-11.In both cultivars,MeJA significantly decreased the contents of ascorbic acid,Rubisco protein and chlorophyll after treatment,while increased the descending rate of photosynthetic parameters,and had a stronger effect on ZH-11than GI-2.Therefore,it was demonstrated that exogenous AsA plays an effective role in alleviating the early-senescence of rice leaf induced by ethephon,while MeJA has the opposite effects.

关 键 词：抗坏血酸 乙烯利 茉莉酸甲酯 水稻 叶片衰老 

分 类 号：S511[农业科学—作物学]