基于重测序的染色体片段代换系定位水稻抽穗期QTL  

Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice

作　　者：王军[1,2] 朱金燕[2] 陶亚军 周勇 范方军[2] 李文奇[2] 王芳权[2] 仲维功[2] 杨杰[2] 梁国华 WANG Jun;ZHU Jinyan;TAO Yajun;ZHOU Yong;FAN Fangjun;LI Wenqi;WANG Fangquan;ZHONG Weigong;YANG Jie;LIANG Guohua(Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Key Laboratory of Plant Function Genomics, Ministry of Education Yangzhou University, Yangzhou 225009, China;Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China)

机构地区：[1]扬州大学江苏省粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心,南京210014

出　　处：《中国水稻科学》2017年第4期364-370,共7页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2013CBA01405);公益性行业科研专项(201303102);江苏省现代农业重点研发项目(BE2015355;BE2015341);扬州市农业前瞻性研究资助项目(YZ2014165)

摘　　要：【目的】水稻的抽穗期是决定水稻产量及其适用性的重要农艺性状之一,是由多基因控制的数量性状。染色体片段代换系减少了个体间遗传背景的干扰,已经成为定位和克隆复杂性状QTL的重要材料。【方法】本研究以9311为受体,日本晴为供体构建的128个重测序的染色体片段代换系群体为试验材料,利用多元回归,结合Bin-map图谱,【结果】鉴定到6个在南京、扬州不同年份间稳定表达的抽穗期QTL,其中,qHD2.1被定位在第2染色体上的759 848 bp区间内;qHD2.2被定位在第2染色体上的45 286 bp区间内;qHD 3.1被定位在第3染色体上的147 931 bp区间内;qHD5.1被定位在第5染色体上的213 351 bp区间内;qHD5.2被定位在第5染色体上的442 305 bp区间内;qHD8.1被定位在第8染色体上的538 176 bp区间内。【结论】本研究为精细定位并克隆相应QTL,进而探明抽穗期QTL的分子调控机制奠定了基础。【Objective】Heading date is an important agronomic trait in rice,it is a typical quantitative trait controlled by multiple genes.As novel research material,chromosome segment substitution lines are useful in QTL fine mapping and cloning because of minimizing the interference of genetic background among plants.【Method】In this study,128whole-genome re-sequenced chromosome segment substitution lines derived from Nipponbare as donor parent in the background of9311,were used for mapping QTLs for heading date by combining the sequencing-based Bin-map with multiple linear regression analysis.【Result】Six QTLs for heading date were identified in two environments and two years.qHD2.1was mapped in the region of759848bp on the chromosome2;qHD2.2was mapped in the region of45286bp on the chromosome2;qHD3.1was mapped in the region of147931bp on the chromosome3;qHD5.1was mapped in the region of213351bp on the chromosome5;qHD5.2was mapped in the region of442305bp on the chromosome5;qHD8.1was mapped in the region of538176bp on the chromosome8.【Conclusion】The results are important for the QTLs cloning and provide a foundation for understanding molecular regulation mechanism of heading date in rice.

关 键 词：水稻 染色体片段代换系 重测序 抽穗期 

分 类 号：S511[农业科学—作物学]