邻氨基苯酚对污泥产率及微生物活性的影响  

Effects of o-aminophenol on sludge yield and microbial activity

作　　者：韦学玉[1,2] 刘志刚[2,3] 闫玉涛[2] 徐晓平[1] 王晓菊 WEI Xue-yu;LIU Zhi-gang;YAN Yu-tao;XU Xiao-ping;WANG Xiao-ju(The School of Civil Engineering and Architecture, Anhui Polytechnic University, Wuhu 241000, China;College of Environment, Hohai University, Nanjing 210098, China;Ningbo Water Supply Co., Ltd,Ningbo 315041, China)

机构地区：[1]安徽工程大学建筑工程学院,安徽芜湖241000 [2]河海大学环境学院,江苏南京210098 [3]宁波市自来水有限公司,浙江宁波315041

出　　处：《中国环境科学》2017年第7期2550-2556,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(51502106);安徽高校自然科学基金重点项目(KJ2017A119)

摘　　要：为了探讨化学解偶联剂在实现污泥减量的同时对污泥活性抑制作用,通过批次试验研究不同浓度邻氨基苯酚(oAP)对污泥减量效果、微生物活性以及由于微生物活性的改变对基质(NH_4^+-N、COD_(Cr))去除变化的影响.分别采用TTC-ETS(氯代三苯基四氮唑脱氢酶)活性、INT-ETS(碘硝基四氮唑脱氢酶)活性、AUR(氨摄取速率)和SOUR(比耗氧速率)4个指标考察污泥活性受oAP的抑制情况.结果表明:oAP添加量为12mg/L时,平均表观污泥产率Yobs由0.443下降到0.256mg MLSS/mg COD,污泥减量为42.20%.与有机物去除抑制相比,活性污泥系统对NH_4^+-N去除的抑制作用更加显著,而硝化细菌比异养菌对oAP响应更敏感.因此,NH_4^+-N去除率比COD_(Cr)去除率更能反映出oAP对污泥活性的影响.通过NH_4^+-N去除率的抑制对比,AUR是最能有效地表征oAP对活性污泥系统中的微生物抑制作用.通过IC50分析显示,TTC-ETS的活性为35.51mg/L,在所有指标最小,灵敏度最高,是表征oAP抑制污泥活性的最佳指标.In order to view sludge reduction and estimate the inhibitory effect of chemical uncoupler on the activity of sludge,the influence of o-aminophenol(oAP)addition on efficiency of sludge reduction.The microbial activity and variation of removal efficiency in NH4+-N and CODCr induced by addition oAP were investigated through batch tests.Four indexes,TTC-electron transport system activity(TTC-ETS),INT-electron transport system activity(INT-ETS),ammonia uptake rate(AUR)and specific oxygen uptake rate(SOUR)were used to characterize the inhibitory effects of the metabolic uncoupler on the sludge activity.The results showed that the average apparent sludge yield of Yobs was decreased from0.443to0.256MLSS/mgCOD for42.20%,when oAP concentration was set as12mg/L.The inhibitory effect of oAP on removing NH4+-N was more significant than on decomposing organics,and nitrifying bacteria were more sensitive than heterotrophic bacteria to the inhibitory effects of oAP,which showed the toxic influence on the activity of sludge.Through comparing the inhibition rates of NH4+-N removal efficiency,AUR is supposed to be the most effective index to characterize the inhibitory effects of metabolic uncoupler.Besides,TTC-ETS activity can be used as the optimum index to characterize inhibitory effects of oAP,because its median inhibitory concentration was the minimum of35.51mg/L,as the minimum one among the four indexes.

关 键 词：化学解偶联剂 邻氨基苯酚(oAP) 污泥活性 氯代三苯基四氮唑脱氢酶活性 碘硝基四氮唑脱氢酶活性 氨摄取速率 比好氧速率 

分 类 号：X703.5[环境科学与工程—环境工程]