水溶性量子点荧光探针用于水产养殖违禁添加品孔雀石绿残留量测定  

Quantitative determination of illegally added malachite green residues in aquaculture using water-soluble quantum dots as fluorescent probes

作　　者：谢晓梅[1] 廖敏[1] 黄宇[1] 叶照金 吕婷[1] XIE Xiaomei;LIAO Min;HUANG Yu;YE Zhaojin;Lü Ting(Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]浙江大学环境与资源学院,浙江省亚热带土壤与植物营养重点实验室,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2017年第3期329-340,共12页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金(41571226,41371312);公益性行业(农业)科研专项(201203045);浙江省测试基金项目(2015C37079)

摘　　要：基于孔雀石绿对水溶性Cd Te/Zn S量子点存在荧光猝灭现象,建立了一种用于水产养殖违禁添加孔雀石绿残留检测的新方法。在p H 8.0、0.065 mol/L的Tris-HCl缓冲溶液及1.60×10^(-4)mol/L的Cd Te/Zn S量子点优化体系中,在0.019 3~1.28 mg/L范围内,孔雀石绿质量浓度与水溶性量子点Cd Te/Zn S的荧光猝灭强度呈良好的线性关系,相关系数r=0.999,检出限为0.005 43 mg/L。该方法可成功用于淡水和海水养殖用水以及鱼肉组织中违禁添加孔雀石绿残留的测定,添加回收率为90.4%~100.3%,相对标准偏差为0.37%~1.01%。该方法对水产品和养殖用水的检测结果与液质联用法的检测结果一致,且假阳性和假阴性率试验结果也均与真实样品一致:说明水溶性量子点荧光探针法对孔雀石绿残留检测特异性较好。通过紫外-可见吸收光谱及共振光散射技术研究孔雀石绿对水溶性Cd Te/Zn S量子点荧光猝灭特征,发现其荧光猝灭机制是Cd Te/Zn S量子点与孔雀石绿相互作用,从而建立起共振能量转移体系。Quantum dots have unique physical and chemical properties and optical properties due to its special structure.In recent years,as a new type of fluorescent probe materials applied in chemical and biological analysis,medical diagnosis and other fields,quantum dots have become the focus of research.At present,some studies have been reported on the detection of heavy metal,veterinary drug and pesticide residues in food by using quantum dots as fluorescent probes.Related research reports showed that the use of quantum dots as a fluorescent probe to analyze the analyte with impact of food safety had advantages of high sensitivity,good selectivity,short response time,and less investment,which indicated the quantum dots as a fluorescent probe had wide application prospects in food safety inspection field.The malachite green had good antibacterial effect and low price,which was often illegally used in aquaculture as insecticides and fungicides.The malachite green had the potential carcinogenic effects,which was clearly listed in Chinese Food Animal Banned Veterinary Drugs and Compounds List in2002,but in fact,malachite green residue has been detected in aquatic products breeding from time to time due to illegal addition.Malachite green residue detection method has been mainly reported,such as high performance liquid chromatography,surface enhanced Raman scattering spectroscopy,liquid chromatography-mass spectrometry(LC-MS),ultraviolet spectrometry,which were not easy to be applied and popularized at the basic unit,due to lower sensitivity,or longer single sample detection time,or expensive equipment,or complicated operation.Therefore,it was urgent to develop a detection method with high sensitivity,good selectivity,short response time and less input.Quantum dot fluorescent probe method in residue detection had the potential to meet the demand,but the current studies using the method of quantum dots to detect malachite green were rarely reported.

关 键 词：水溶性量子点 荧光探针 孔雀石绿 荧光猝灭 

分 类 号：R917[医药卫生—药物分析学]