水稻颖花持续开放突变体sostenuto floret opening(sfo1)的鉴定与基因定位  

Characterization and Gene Mapping of sostenuto floret opening 1(sfo1) Mutant in Rice(Oryza sativa L.)

作　　者：沈亚林[1] 庄慧[1] 陈欢[1] 曾晓琴[1] 李香凝[1] 张君[1] 郑昊[1] 凌英华[1] 李云峰[1] SHEN Ya-Lin;ZHUANG Hui;CHEN Huan;ZENG Xiao-Qin;LI Xiang-Ning;ZHANG Jun;ZHENG Hao;LING Ying-Hua;LI Yun-Feng(Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400716, China)

机构地区：[1]西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室,重庆400716

出　　处：《作物学报》2017年第8期1122-1127,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31271304);中央高校基本科研业务费(XDJK2016A013)资助~~

摘　　要：水稻颖花开放是其生殖发育一个关键生理过程,对受精和随后种子发育具有重要影响。本文报道了一个与水稻颖花开放相关的突变体,来源于籼稻保持系西农1B的EMS(ethyl methane sulfonate)诱变群体。该突变体表现为开颖后浆片失水萎缩过程缓慢,内外稃持续开裂不闭合,暂命名为水稻颖花持续开放sostenuto floret opening 1(sfo1)突变体。遗传分析表明sfo1性状受1对隐性单基因控制,利用群体分离分析法(bulked segregation analysis,BSA)将SFO1基因定位在第5染色体SSR标记RM1054和IN/DEL标记ZTQ51之间,物理距离113 kb,含注释基因15个。本研究结果为SFO1基因的图位克隆和功能研究奠定了基础。Rice floret opening is one of the most critical physiological processes in its reproductive development,which has asignificant influence on the fertilization and subsequent seed development.Rice floret opening and closing are promoted respectivelyby the lodicules’expansion and shrink.In recent years,many studies focused on the molecular mechanism of rice lodiculedevelopment.However,few studies focused on the molecular regulation mechanism in physiological process of lodicule openingand closing.In this paper,we reported a floret opening mutant,sostenuto floret opening1(sfo1),derived from EMS(ethyl methanesulfonate)mutation groups of Xinong1B.During the floret opening stage,the florets of sfo1and wild type were observeddirectly by both the stereoscope and the scanning electron microscopy at about1h before opening,and10min,1.5h and48hafter opening respecting.Compared with that in the wild type,the sfo1floret showed a delay of lodicules dehydration after floretopening,resulting in its failing to close the lemma and palea.Significant differences were not found at1h before opening,showingclosed hulls,flat lodicules and well-stacked lodicules surface cells.At10min after opening,there were also no obvious differencesbetween wild type and mutants,showing opening hulls,inflated lodicules,and smooth and well-stacked surface cells.At1.5h after opening,sfo1hulls were not closed so big as those in the wild type.And sfo1lodicules still inflated,but not dehydratedand atrophied as those in the wild type.At48h after opening,sfo1hulls were not closed yet,its lodicules began to dehydrate andatrophy,but were still very full compared with those in the wild type.The F1and F2of a cross with sterile lines56S as femaleparent and the sfo1as male parent were used in genetic analysis and gene mapping,indicating that the sfo1trait was controlled bya nuclear recessive gene.Using bulked segregation analysis(BSA)method,the SFO1was located between the SSR markerRM1054and Insert/Delete marker ZTQ51on the chromosome5,with a physical distance of11

关 键 词：水稻 颖花开放 浆片 基因定位 

分 类 号：S511[农业科学—作物学]