普通菜豆籽粒大小与形状的QTL定位  

QTL Mapping for Seed Size and Shape in Common Bean

作　　者：耿庆河 王兰芬[1] 武晶[1] 王述民[1] GENG Qing-He;WANG Lan-Fen;WU Jing;WANG Shu-Min(Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2017年第8期1149-1160,共12页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31471559);国家现代农业产业技术体系建设专项(CARS-09);国家科技支撑计划项目(2013BAD01B05-2-4)资助~~

摘　　要：普通菜豆是世界上最重要的食用豆类作物之一,其籽粒大小和形状与产量及外观品质密切相关。本研究以来自安第斯基因库的大粒品种龙270709和来自中美基因库的小粒品种F5910配置杂交组合,获得的F2分离群体分别在哈尔滨大田与北京昌平温室种植,对百粒重、粒长、粒宽、粒厚、长宽比和长厚比6个籽粒性状进行了相关性分析和QTL定位。相关性分析表明,百粒重与粒长、粒宽、粒厚、长宽比、长厚比5个衡量籽粒大小和形状的性状均显著正相关。利用基于完备区间作图方法的Ici Mapping 4.1进行QTL定位,哈尔滨环境下定位到38个与百粒重、粒长、粒宽、粒厚、长宽比、长厚比相关的QTL,表型贡献率介于2.39%~17.37%之间,分布在除第1染色体外的其余10条染色体上;北京昌平环境下定位到21个上述性状的QTL,表型贡献率介于5.92%~22.53%之间,分布在第1、第3、第6、第7、第8、第9和第11染色体上。其中,百粒重QTLSW7与SW7’,SW6.1与SW6’,粒长QTLSL6.1与SL6.1’,粒厚QTLSH11与SH11’在2个环境下的标记区间重叠或者重合,SW7、SW6.1、SL6.1、SW6’和SL6.1’的表型贡献率在10%以上。Common bean is one of the most important food legumes worldwide.Seed size and shape have a great effect on yieldand seed quality.This research used an F2population derived from a cross of Andean cultivar Long270709?Mesoamerican cultivarF5910planted in Harbin,Heilongjiang and Beijing respectively to analyze the correlation among seed weight,seed length,seed width,seed height,seed length-to-width ratio and seed length-to-height ratio,and to detect QTL related to these traits.Thecorrelation analysis showed that seed weight had a significant correlation with other traits related to seed size and shape.SoftwareIciMapping4.1based on inclusive composite interval mapping was used to identify the additive QTL for seed size and shape.Atotal of38QTL were detected on10chromosomes except the first chromosome in Harbin environment with the PVE between2.39%and17.37%.A total of21QTL were detected on seven chromosomes(Chr.1,Chr.3,Chr.6,Chr.7,Chr.8,Chr.9,Chr.11)inBeijing environment with the PVE between5.92%and22.53%.Among them,four pairs of QTL were detected on the samemarker interval,including seed weight QTL SW7and SW7’,seed weight QTL SW6.1and SW6’,seed length QTL SL6.1andSL6.1’,and seed height QTL SH11and SH11’.The PVE of SW7,SW6.1,SL6.1,SW6’,and SL6.1’was larger than10%.

关 键 词：普通菜豆 籽粒大小 粒形 数量性状位点(QTL) 

分 类 号：S643.1[农业科学—蔬菜学]