广西地区胱抑素C及其单核苷酸多态性与冠心病的关系  

Relationship of cystatin C and single nucleotide polymorphisms with coronary heart disease in Guangxi

作　　者：黄胜贤[1] 郑利平[1] 杨兰[1] 陆俊佳[1] 朱旭[1] HUANG Shengxian;ZHENG Liping;YANG Lan;LU Junjia;ZHU Xu(Nanning Second People's Hospital,Nanning 530031,Guangxi,China)

机构地区：[1]南宁市第二人民医院,广西南宁530031

出　　处：《检验医学》2017年第7期597-602,共6页Laboratory Medicine

基　　金：广西壮族自治区南宁市科技课题(2012137)

摘　　要：目的探讨广西地区胱抑素C(Cys C)水平及其基因位点+73A/G和-82G/C多态性与冠心病(CHD)的相关性。方法选择壮族CHD患者、壮族体检健康者(壮族正常对照组)、汉族CHD患者和汉族体检健康者(汉族正常对照组)各100例,采用免疫比浊法检测血清Cys C水平,聚合酶链反应(PCR)-限制性片段长度多态性(RFLP)检测Cys C+73A/G和-82G/C位点的基因多态性,同时收集一般临床资料。结果CHD组与同民族正常对照组之间血清Cys C水平及一般临床资料差异均有统计学意义(P<0.05);CHD组Cys C+73A/G位点基因型频率与同民族正常对照组比较,差异均有统计学意义(P<0.05),且GG基因型的CHD患者Cys C水平明显低于AG和AA型(P<0.05),而AG与AA基因型之间差异无统计学意义(P>0.05)。CHD组血清Cys C水平与肌酐(Cr)水平、Cys C+73A/G基因位点呈正相关(r=0.60,P<0.01;r=0.42,P<0.01)。结论 CHD患者肾功能下降引起的Cys C水平升高掩盖了Cys C+73A/G基因多态性所致的低Cys C水平。Cys C+73A/G基因多态性所致的低Cys C水平可能是广西地区人群CHD的易感因素。Objective To investigate the relationship of cystatin C(Cys C)and+73A/G and-82G/C single nucleotide polymorphisms with coronary heart disease(CHD)in Guangxi.Methods Serum levels of Cys C in Zhuang CHD patients,Zhuang healthy subjects,Han CHD patients and Han healthy subjects(100cases for each group)were determined by immunoturbidimetric assay,and+73A/G and-82G/C single nucleotide polymorphisms were determined by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP).The clinical information was collected.Results Serum Cys C levels and clinical information between CHD patients and healthy subjects had statistical significance(P<0.05).The frequency of Cys C+73A/G polymorphism in CHD patients had statistical significance with that in healthy subjects(P<0.05).Serum Cys C levels in CHD patients with GG genotype were lower than those with AG and AA genotypes(P<0.05).There was no statistical significance between AG and AA genotypes(P>0.05).Serum levels of Cys C had positive correlations with creatinine(Cr)levels and Cys C+73A/G polymorphism in CHD patients(r=0.60,P<0.01;r=0.42,P<0.01).Conclusions The increasing of serum Cys C levels caused by the decreasing of renal function covers the low levels of Cys C caused by Cys C+73A/G polymorphism.The polymorphism of Cys C+73A/G Polymorphism resulting low-level Cys C may be a risk factor for CHD in Guangxi.

关 键 词：胱抑素C 基因多态性 汉族 壮族 冠心病 

分 类 号：R446.1[医药卫生—诊断学]