四种口蹄疫病毒非结构蛋白抗体ELISA试剂盒检测猪血清样本的质量评估  

Evaluation on the Performance of Four ELISA Tests for Antibody against Non-structural Protein of Foot-and-mouth Disease in Swine Sera

作　　者：史茜[1] 陈瑞花[1] 王科珂 徐新峰[1] 卢曾军[2] 季新成[1] Shi Qian;Chen Ruihua;Wang Keke;Xu Xinfeng;Lun Zengjun;Ji Xincheng(Xinjiang Entry-exit Inspection and Quarantine Bureau,Urumqi,Xinjiang 830063;Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science,Lanzhou,Gansu 730046)

机构地区：[1]新疆出入境检验检疫局,新疆乌鲁木齐830063 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《中国动物检疫》2017年第8期100-103,113,共5页China Animal Health Inspection

基　　金：国家质检总局质检公益专项(201410061)

摘　　要：[目的]对4种口蹄疫病毒非结构蛋白抗体ELISA试剂盒进行质量评估。[方法]用4种商品化的口蹄疫非结构蛋白抗体ELISA检测试剂盒,对非免疫无疫、免疫健康、免疫感染和非免疫人工感染4种类型猪血清样本进行检测,分析试剂盒的诊断敏感性、诊断特异性、分析敏感性、分析特异性及重复性。[结果]4种试剂盒的诊断敏感性在76.8%~94.4%之间,诊断特异性在97.7%~100%之间,分析敏感性和分析特异性较好。4种检测试剂盒与4种猪常感染疾病的阳性血清不发生反应。试剂盒的批间重复性为(10.545±6.845)%^(37.577±28.626)%;批内重复性为(9.037±6.075)%^(37.601±27.027)%。[结论 ]在实际应用中,需根据检测目的不同,选取相应的试剂盒。[Objective]To evaluate the quality of four commercialized ELISA kits for the detection of antibodies to thenon-structural proteins(NSPs)of Foot-and-mouth disease virus(FMDV).[Methods]The four commercializedELISA kits were compared.The diagnostic sensitivity and specificity,analytic sensitivity and specificity,andrepeatability of FMDV NSP ELISA kits were analyzed by detecting four types of sera from uninfected,vaccinated,naturally infected and challenged swine.[Results]The diagnostic sensitivity of the four ELISA kits were between76.8%to94.4%,the relatively high diagnostic specificity were ranging from97.7%to100%,and both analytic sensitivityand specificity were good.Sera from swine infected with other four different diseases cannot be detected using the kits.The repeatability of intra-assay was between(10.545±6.845)%to(37.577±28.626)%,and the inter-assay wasbetween(9.037±6.075)%to(37.601±27.027)%.[Conclusion]In practice,the different kits could be selectedfor different detection purpose.

关 键 词：口蹄疫 非结构蛋白抗体 ELISA试剂盒 质量评估 

分 类 号：S851.33[农业科学—预防兽医学]