氧化芍药苷对泡沫细胞胆固醇流出的影响  

Effect of Oxypaeoniflora on cholesterol efflux from foam cells

作　　者：唐菀泽[1] 马卫列[1] 丁航[1] 向乐[1] 张志珍[1] Wan-ze Tang;Wei-lie Ma;Hang Ding;Le Xiang;Zhi-zhen Zhang(Department of Biochemistry and Molecular Biology, Guangdong Medical University, Dongguan,Guangdong 523808, China)

机构地区：[1]广东医科大学生物化学与分子生物学教研室,广东东莞523808

出　　处：《中国现代医学杂志》2017年第16期6-11,共6页China Journal of Modern Medicine

摘　　要：目的分析氧化芍药苷对THP-1巨噬细胞源性泡沫细胞胆固醇流出的影响。方法 THP-1细胞加入160 nmol/L佛波酯(PMA)24 h,再加入50μg/ml乙酰化低密度脂蛋白(ac-LDL)建立泡沫细胞模型,CCK-8法测定氧化芍药苷的细胞毒性,同位素标记法测定各处理组泡沫细胞胆固醇流出率。共聚焦显微镜观察泡沫细胞内脂肪分化相关蛋白(ADFP)表达变化,Western blot分析泡沫细胞中ADFP、三磷酸腺苷结合盒转运体A1(ABCA1)和过氧化物酶体增殖物激活受体α(PPARα)蛋白表达变化。结果成功建立THP-1巨噬细胞源性泡沫细胞模型。氧化芍药苷毒性分析表明,质量浓度在200.0μg/ml以下无细胞毒性。150.0μg/ml与200.0μg/ml氧化芍药苷处理泡沫细胞,胆固醇流出率分别为(10.317±0.508)%与(11.265±0.713)%,与apo A-1组相比,差异有统计学意义(P<0.05),均高于apo A-1组。氧化芍药苷处理的泡沫细胞内ADFP荧光明显减弱。Western blot分析泡沫细胞ADFP表达量降低28%。氧化芍药苷处理组PPARα表达量增加51%,ABCA1表达量增加45%。结论实验表明氧化芍药苷可通过上调PPARα增加ABCA1表达,进而促进泡沫细胞胆固醇流出。Objective To analyze the effect of Oxypaeoniflora on cholesterol efflux from THP-1macrophagederivedfoam cells.Methods THP-1cells were treated with160nmol/L phorbol-1-myristate-13-acetate(PMA)for24h,then50滋g/ml acetylated low-density lipoprotein(ac-LDL)to establish foam cell model.Cytotoxicity ofOxypaeoniflora was assessed by CCK-8.Cholesterol efflux rate of the foam cells in each treatment group wasevaluated by isotope labeling method.The expression of adipose differentiation related protein(ADFP)in the foamcells was observed by confocal laser scanning microscopy.The expression changes of ADFP,ATP binding cassettetransporter A1(ABCA1)and peroxisome proliferator-activated receptor alpha(PPAR琢)in the foam cells weredetected using Western blot.Results The THP-1macrophage-derived foam cell model was successfully established.At the concentration of lower than200.0滋g/ml,Oxypaeoniflora was not cytotoxic.When the Oxypaeonifloraconcentrations were150.0滋g/ml and200.0滋g/ml,the cholesterol efflux rates were about(10.317依0.508)%and(11.265依0.713)%respectively,which were increased compared with the control group(<0.05).Undermicroscope,the fluorescence of ADFP in the Oxypaeoniflora groups was obviously decreased.Western blot indicatedthat the expression of ADFP was decreased by28%,while the expressions of PPAR琢and ABCA1were increased by51%and45%respectively.Conclusions The results state clearly Oxypaeoniflora can increase the expression ofABCA1by up-regulation of PPAR琢expression and then promote the cholesterol efflux from foam cells.

关 键 词：氧化芍药苷 THP-1巨噬细胞源性泡沫细胞 胆固醇流出 

分 类 号：R282[医药卫生—中药学] Q54[医药卫生—中医学]