碱性成纤维细胞生长因子在慢性硬膜下血肿患者外膜中的表达  

Expression of basic fibroblast growth factor in the adventitia of patients with chronic subdural hematoma

作　　者：汪新宇[1] 陈晓旭[1] 姜大宇[1] 李国栋[1] 董克辛[1] WANG Xin-yu;CHEN Xiao-xu;JIANG Da-yu(Department of Neurosurgery, Dalian City Friendship Hospital, Dalian 116001, China)

机构地区：[1]大连市友谊医院神经外科,116001

出　　处：《中国现代药物应用》2017年第15期18-19,共2页Chinese Journal of Modern Drug Application

基　　金：大连市卫生局课题项目(项目编号:WSJ/KJC-01-JL-01)

摘　　要：目的通过检测碱性成纤维细胞生长因子(b FGF)在慢性硬膜下血肿(CSDH)外膜中的表达,研究其与CSDH病情发展的关系。方法选取32例CSDH患者,术中取材血肿外膜及硬膜,通过免疫组化检测b FGF在CSDH患者血肿外膜及硬膜的表达。结果 CSDH外膜为新鲜肉芽组织,可见网状血管存在,其间为中性粒细胞、嗜酸性粒细胞、成纤维细胞、浆细胞浸润并聚集,相邻硬膜未见明显异常。高倍显微镜下观察发现b FGF主要表达在中性粒细胞、嗜酸性粒细胞、成纤维细胞、浆细胞的胞浆中,而在邻近硬膜中未见明显表达;血肿外膜b FGF为强阳性表达,术后复发7例,占22%,未复发25例,占78%,则术后血肿复发危险性明显增加,差异具有统计学意义(P<0.05)。结论 b FGF来源于血肿外膜中的中性粒细胞、嗜酸性粒细胞、成纤维细胞、浆细胞,其介导血肿外膜新生血管形成、局部炎症反应,最终导致血肿量增多,病情逐渐加重。Objective To detect the expression of basic fibroblast growth factor(bFGF)in adventitiaof patients with chronic subdural hematoma(CSDH),and study its correlation with CSDH disease progression.Methods A total of32patients with CSDH were enrolled in this study.The hematoma adventitia and duramater were collected and the expression of bFGF in hematoma adventitia and dura mater of CSDH patients wasdetected by immunohistochemistry.Results CSDH adventitia is fresh granulation tissue,showing the presence ofreticular vessels,among which were neutrophils,eosinophils,fibroblasts,plasma cell infiltration and aggregation.No obvious abnormality exists in adjacent dura mater.Under high power microscope,bFGF was mainly expressedin the cytoplasm of neutrophils,eosinophils,fibroblasts and plasma cells,but no obvious expression was observedin the adjacent dura mater.The hematoma adventitia bFGF of hematoma was strongly positive,and there were7recurrence cases after operation,accounting for22%and25non-recurrence cases,accounting for78%.The riskof postoperative recurrence of hematoma increased significantly,and the difference had statistical significance(P<0.05).Conclusion BFGF is derived from hematoma adventitia of neutrophils,eosinophils,fibroblasts,plasmacells,which mediates hematoma epicardial neovascularization,local inflammatory response,eventually leading toincreased hematoma number and gradually worsened condition.

关 键 词：碱性成纤维细胞生长因子 慢性硬膜下血肿 

分 类 号：R651.1[医药卫生—外科学]