脊柱结核患者特异循环miRNA表达的初步研究  

A preliminary study on the expression of specific circulating miRNA in patients with spinal tuberculosis

作　　者：黄志刚[1] 马克[1] 王自立[2] 刘晶[1] 

机构地区：[1]深圳市第三人民医院,广东深圳518112 [2]宁夏医科大学总医院,宁夏银川750004

出　　处：《中医临床研究》2017年第18期92-95,共4页Clinical Journal Of Chinese Medicine

基　　金：深圳市科技计划项目资助(编号:JCYJ20160427152847715).

摘　　要：目的:研究探寻对脊柱结核有潜在诊断价值的特异循环mi RNA标记物。方法:以20例脊柱结核患者作为实验组,20例健康组作为对照组,分别提取外周血血清中RNA,进行mi RNA基因芯片检测,筛选出差异表达的mi RNA,并用实时荧光定量PCR进行验证。结果:通过mi RNA基因芯片初步筛选出17个表达有差异的mi RNAs,其中1个表达上调,16个表达下调。经过实时定量PCR验证,与对照组相比,有5个mi RNAs表达量显著异常,其中表达下调mi RNA(P<0.05)为:hsa-mi R-30c-5p、hsa-mi R-432-5p、hsa-mi R-192-5p、hsa-mi R-532-5p,表达上调mi RNA(P<0.05)为hsa-mi R-210-3p。结论:脊柱结核患者血清中有一些特异性循环mi RNA表达,其可能成为潜在的有诊断价值的标记物。Objective:To investigate the expression of specific circulating miRNAs in the peripheral blood of spinal tuberculosispatients.Methods:After isolation of total RNAs from the serum of spinal tuberculosis patients(n=20)and healthy control group patients(n=20)respectively,Microarrays were used to detect the differently expressed microRNAs.The qRT-PCR verified the findings frommicroarray screening.Students’t-test was applied to screen out the microRNAs with diverse expression levels.Results:Analysis ofexpression profiles indicated that the expression of17microRNAs was obviously varied in the serum from spinal tuberculosis patientscompared with that of the healthy control group.One of them was up-regulated,and other microRNAs were down-regulated.qRT-PCRvalidated that hsa-miR-30c-5p,hsa-miR-432-5p,hsa-miR-192-5p and hsa-miR-532-5p were down-regulated expressed(P<0.05),while hsamiR-210-3p was significantly up-regulated in the spinal tuberculosis patients(P<0.05).Conclusion:There were some specific circulatingmicroRNA expressions in peripheral blood of patients with spinal tuberculosis and these microRNAs might be considered as potentialbiomarkers for spinal tuberculosis diagnosis.

关 键 词：微小RNA 结核 脊柱 诊断 标记物 

分 类 号：R68[医药卫生—骨科学]