抑芽丹在烟草上的消解趋势及安全性评价  

Degradation Trend and Safety Evaluation of Maleic Hydrazide in Tobacco Leaves

作　　者：郑晓[1] 徐金丽[1] 徐光军[1] ZHENG Xiao;XU Jinli;XU Guangjun(Tobacco Research Institute of CAAS, Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management Qingdao 266101, China)

机构地区：[1]中国农业科学院烟草研究所,国家烟草专卖局病虫害监控与综合治理重点实验室,青岛266101

出　　处：《中国烟草科学》2017年第3期51-55,共5页Chinese Tobacco Science

摘　　要：为评价抑芽丹在烟草上的消解趋势及安全性,建立了一种简单可行的残留量测定方法,并开展两年两地消解动态和最终残留研究。结果表明,烟草以酸化甲醇提取,SPE-C18固相萃取小柱净化,高效液相色谱测定,抑芽丹最低检测浓度为0.1 mg/kg。在1.0~80.0 mg/kg添加水平下,抑芽丹在鲜烟叶和干烟叶中的平均回收率分别为81.81%~95.89%和82.01%~83.08%,相对标准偏差分别为2.39%~7.03%和1.04%~1.71%。在消解动态试验施药后21 d,抑芽丹在烟草上的消解率超过90%,理论半衰期7.9~9.6 d,消解趋势符合一级动力学方程;施药后7 d,抑芽丹最终残留量低于80 mg/kg的指导性残留限量标准,安全风险处于较低水平。To evaluate the degradation trend and safety of maleic hydrazide in tobacco leaves,a simple and feasible residue analytical method was established,which was used in the study of digestion dynamics and final residues for2years.Maleic hydrazide in tobacco leaf samples was extracted with acid methanol and purified on C18solid phase extraction column.The results showed that the limit of quantification(LOQ)was0.1mg/kg by the determination of high performance liquid chromatography.At spiked levels of1.0-80.0mg/kg,the average recoveries ranged from81.81%to95.89%for green tobacco leaves and from82.01%to83.08%for dried tobacco leaves,with relative standard deviations(RSD)of2.39%-7.03%and1.04%-1.71%,respectively.The results of maleic hydrazide degradation dynamic tests showed that the digestion rate in green tobacco was over90%after21days of treatment,with the theoretical half-life being7.9-9.6days and the digestion trend was in line with the first order kinetic equation.Maleic hydrazide residues after7days of spraying were below the MRL of80mg/kg.The residual amount of maleic hydrazide in tobacco was at a lower level of safety risk.

关 键 词：抑芽丹 烟草 残留 消解趋势 安全性 

分 类 号：S481.8[农业科学—农药学]