小麦F-box基因TaFBL14的克隆及表达分析  

Cloning and Expression Analyses of F-box Gene TaFBL14 in Wheat

作　　者：李铃仙 魏春茹[1,2] 李虎滢 魏新燕 于秀梅[1,2,3] LI Lingxian;WEI Chunru;LI Huying;WEI Xinyan;YU Xiumei(College of Life Sciences,Hebei Agricultural University,Baoding,Hebei 071001,China;Key Laboratory of Hebei Province for Molecular PlantMicrobe Interaction,Baoding,Hebei 071001,China;Biological Control Centre of Plant Disease and Plant Pests of Hebei Province,Baoding,Hebei 071001,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北省植物生理与分子病理学重点实验室,河北保定071001 [3]河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《西北植物学报》2017年第2期232-238,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31301649)

摘　　要：该研究利用同源克隆策略,获得了1条小麦F-box/LRR重复蛋白14基因(TaFBL14)。TaFBL14基因编码486个氨基酸,预测分子量为53.48kD,等电点为5.93,序列N端包含一个F-box结构域,C段包含7个LRR结构域。TaFBL14基因编码蛋白不具有信号肽及核定位信号序列,主要定位在细胞质中,二级结构以α-螺旋为主,呈球状。进化树分析表明,TaFBL14与粗山羊草和乌拉尔托小麦的FBL14蛋白亲缘关系较近。qRT-PCR分析结果显示,TaFBL14基因主要在小麦叶组织中表达,且受非亲和叶锈菌侵染后呈现上调表达趋势,说明该基因可能参与小麦抵御叶锈菌的侵染过程。Using the homologybased cloning,we obtained a F-box/LRR repeat protein14gene(TaFBL14)from wheat in the present study.TaFBL14ncoded a486aa polypeptide with predicted molecular weight of53.48kD and theoretical isoelectric point5.93.The deduced protein included a F-box domain in the Nterminal and7LRR domains in the C terminal.No signal peptide and nuclear localization sequence were detected.Subcellular localization showed TaFBL14mainly expressed in the cytoplasm.αhelix was the most important secondary structure,which made TaFBL14the globular protein.Neighbour joining tree showed that the deduced TaFBL14protein shared high similarity with FBL14fromAegilops tauschii nd riticum urartu.Quantitative reverse transcription PCR showed that TaFBL14mainly expressed in wheat young leaves and flag leaves,and were up regulated expression from6hpi to96hpi.These results implied that TaFBL14may be involved in wheat resistant to leaf rust pathogen infection.

关 键 词：小麦 F-box基因TaFBL14 序列分析 表达模式 

分 类 号：Q785[生物学—分子生物学] Q786