根施甜菜碱对镍胁迫下小麦幼苗生长生理的影响  

Physiological Effects of Root-applying Glycine Betaine on Wheat Seedlings Growth under Nickel Stress

作　　者：巴青松[1] 张根生[1] 凌玉[1] 李桂萍[1] 宋运贤[1] 张鹏飞[2] 傅兆麟 BA Qingsong;ZHANG Gensheng;LING Yu;LI Guiping;SONG Yunxian;ZHANG Pengfei;FU Zhaolin(College of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei, Anhui, 235000, China;Xiangyang Academy of Agricultural Sciences, Xiangyang, Hubei, 441057, China)

机构地区：[1]淮北师范大学生命科学学院,资源植物生物学安徽省重点实验室,安徽淮北235000 [2]襄阳市农业科学院,湖北襄阳441057

出　　处：《西北植物学报》2017年第2期315-320,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：安徽省自然科学基金(1708085QC62);安徽省高等学校省级自然科学研究项目(KJ2014B19;KJ2016A624;KJ2013A232);淮北师范大学博士科研启动基金(15600971;12601033)

摘　　要：以普通小麦品种‘轮选988’为材料,采用溶液培养法,研究了根施不同浓度甜菜碱(1.0、2.0、3.0、4.0、5.0、10.0、15.0、20.0mmol·L^(-1))对镍(100μmol·L^(-1) NiSO_4)胁迫下小麦根系生长的影响,以及4.0mmol·L^(-1)甜菜碱处理镍胁迫幼苗根系相关抗逆生理生化指标的变化。结果表明:(1)与不施加镍对照相比,镍胁迫下小麦幼苗的根长、株高、鲜重和干重分别显著降低了14.7%、11.7%、15.0%和16.7%。(2)与单独镍胁迫处理相比,小麦幼苗的根长、株高、鲜重和干重均随着根施甜菜碱的浓度逐渐增加且呈先升后降的趋势,并以4.0mmol·L^(-1)外源甜菜碱处理效果较佳。(3)与单独镍胁迫处理相比较,在4.0mmol·L^(-1)外源甜菜碱处理下,小麦幼苗根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性分别升高了284.7%、40.3%、82.9%和20.4%,超氧阴离子自由基(O-·2)含量、过氧化氢(H_2O_2)含量和丙二醛(MDA)含量分别显著降低了50.6%、38.4%和40.6%,可溶性糖含量及游离脯氨酸(Pro)含量分别显著降低了19.2%、45.4%,而根系活力大幅上升了358.0%。研究认为,根施适宜浓度外源甜菜碱可显著增强小麦幼苗根系的抗氧化能力,恢复根系活力,从而有效减弱镍胁迫对小麦幼苗生长的伤害。To explore the influence of the wheat seedling growth under nickel stress with exogenous betaine was studied the effects of rootapplying glycinebetaine(GB)with1.0,2.0,3.0,4.0,5.0,10.0,15.0,20.0mmol·L-1treatments with100μmol·L-1NiSO4.And the physiological and biochemical indexes of stress resistance under the stress of100μmol·L-1NiSO4by solution culture method.The results indicated that:(1)under the stress of100μmol·L-1of NiSO4,the root length,seedling height,fresh weight and dry weight decreased significantly by14.7%,11.7%,15.0%and16.7%,respecticely,as compared with the control.(2)With the increasing concentration of applied glycinebetaine,the root length,seedling height,fresh weight and dry weight increased at first and then descended,compared with the100μmol·L-1of NiSO4.And the effect of exogenous GB with4.0mmol·L-1treatment was better.(3)Under the concentration of4.0mmol·L-1of GB,the activities of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and ascorbate perxoidase(APX)in roots increased by284.7%,40.3%,82.9%and20.4%,respectively;Superoxide anion(O-2),hydrogen peroxide(H2O2)and malondialdehyde(MDA)contents decreased by50.6%,38.4%and40.6%,respectively;soluble sugar and free proline contents decreased by19.2%,45.4%,respectively;Root activity significantly increased by358.0%,compared with the100μmol·L-1of NiSO4.Therefore,the appropriate concentration of root applying GB could significantly enhance the antioxidant capacity in root of wheat seedlings,and restore the root activity,so as to effectively reduce the harm of nickel stress on the growth of wheat seedlings.

关 键 词：甜菜碱 镍胁迫 小麦根系 生理性状 

分 类 号：Q945.78[生物学—植物学]