肺炎支原体IgM抗体和DNA检测在儿童肺炎支原体感染诊断中的应用  

Application of Mycoplasma pneumoniae IgM antibody and DNA detection in the diagnosis of Mycoplasma pneumoniae infection in children

作　　者：李生梅[1] 黄姗[2] 鲁艳军[3] 王雄[3] 沈娜[3] 刘为勇[3] 朱耀武[3] 彭静[3] LI Shengmei;HUANGShan;LU Yanjun;WANG Xwng;SHEN Na;LIU Weiyong;ZHU Yaowu;PENG Jing(Department of Clinical Laboratory,Qinhai Red Cross Hospital ,Xining ,Qinghai 810000, China;Department o f Pediatrics, Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan , Hubei 430030 , China;Department of Clinical Laboratory , Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology , Wuhan , Hubei 430030 ,China)

机构地区：[1]青海红十字医院检验科,西宁810000 [2]华中科技大学同济医学院附属同济医院儿科,武汉430030 [3]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《国际检验医学杂志》2017年第A01期1-4,共4页International Journal of Laboratory Medicine

基　　金：国家科技重大专项“武汉及周边城市五大症候群病原体常规监测及细菌耐药网研究”(2017ZX10103005-007)资助.

摘　　要：目的比较两种肺炎支原体（MP)检测方法的诊断效能并分析湖北地区MP感染的流行病学特征。方法回顾分析1631例肺炎患儿的咽拭子MP-DNA和血清MP-IgM抗体检测结果。结果确诊为MP感染的患儿有470例，占28.82%(470/1631);MP-IgM抗体和MP-DNA的阳性检出率分别为70.00%(329/470)和56.38%(265/470)，差异有统计学意义（P<0.01);MP-IgM、MP-DNA同时阳性患者占26.38%(124/470);MP感染全年均可发生，其中第四季度感染率最高（39.51%，177/448);MP在女童中的检出率明显高于男童，差异有统计学意义（P<0.05);MP检出率在各年龄组间有明显差异（P<0.01)，其中4?7岁组检出率最高（59.85%)，<1岁组最低（18.60%)。结论联合MP-IgM和MP-DNA检测对提高MP感染检出率有重要意义。Objective To compare the diagnostic efficacy of two Mycoplasma pneumoniae(MP)detection methods and to analyze the epidemiological characteristics of MP infection in Hubei.Methods The test results of throat swab MP-DNA and serum MP-IgM antibody of1631children with pneumonia were analyzed retrospectively.Results A total of470children were comfirmed as MP infection,accounted for28.82%(470/1631).The positive rates of MP-IgM antibody and MP-DNA were70%(329/470)and56.38%(265/470)respectively,and the difference was statistically significant(P<Co.01).MP-IgM and MP-DNA positive patients accounted for26.38%(124/470).MP infection occurred in the whole year which was most prevalent in the fourth quarter(39.51%,177/448).The detection rate of MP in girls was significantly higher than that in boys,the difference was statistically significant(P<C0.05).The detection rate of MP was significantly different among different age groups(P<C0.01),among which the detection rate was the highest in the4-7year old group(59.85%).Conclusion The combination of MP-IgM and MP-DNA detection is of great significance to improve the detection rate of MP infection.

关 键 词：肺炎支原体 DNA检测 流行病学 

分 类 号：R725.631[医药卫生—儿科]