两种血细胞分析方法的比较  

Comparison of two methods to blood cells analysis

作　　者：杜建明[1] 周双艳[1] 杨泽华[1] DU Jianming;ZHOU Shuangyan;YANG Zehua(Department of Clinical Laboratory,First Hospital of Shanxi Medical University,Taiyuan,Shanxi030001,China)

机构地区：[1]山西医科大学第一医院检验科,太原030001

出　　处：《国际检验医学杂志》2017年第A01期56-58,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨BeckmanCoulterDXH-800血细胞分析仪（光散射原理）和ABXPENTRA-DF-120血细胞分析仪（双鞘流系统原理）在血细胞检测结果中的相关性和可比性。方法参考美国临床和实验室标准协会的EP9-A2文件，比较红细胞(RBC)、白细胞（WBC)、血红蛋白（Hb)、血细胞比容（HCT)、血细胞平均体积（MCV)、平均血红蛋白量（MCH)、平均血红蛋白浓度（MCHC)、血小板（PLT)及白细胞五分类检测结果。结果RBC、WBC、Hb、PLT和白细胞五分类（除嗜碱性粒细胞外）结果相关系数r>0.975,差异无统计学意义（P>0.05);HCT、MCV、MCH、MCHC和嗜碱性粒细胞结果相关系数厂<0.975,差异有统计学意（P<〇.〇5h结论实验室应用不同的检测方法检测血细胞分析样本时，应定期进行方法比对和偏倚评估，评价其检测结果的相关性和可比性，并建立相应的显微镜复检规则弥补仪器检测的不足，以保证为临床诊疗疾病提供准确、一致。Objective To investigate the correlation and comparability of Beckman Coulter DXH-800blood cell analyzer(light scattering principle)and ABX PENTRA-DF-120blood cell analyzer(double sheath flow system principle)in blood cell test results Methods Acording to the Clinical and Laboratory Standards Institute EP9-A2guidelines,the test results of red blood cell(RBC),white blood cell(WBC),hemoglobin(Hb),hematocrit(HCT),the average volume of blood cells(MCV),mean hemoglobin(MCH),mean hemoglobin concentration(MCHC),platelet(PLT)and leukocyte five-classification were compared.Results The correlation coefficient r of RBC,WBC,Hb,PLT and leukocyte five-classification(except basophils outside)was^O.975,the differ-ence was not statistically significant(P>0.05);the correlation coefficient r of HCT,MCV?MCH,MCHC and basophil was<C0.975,the difference was statistically significant(P<C〇.05).Conclusion When the laboratory uses different detection methods to detect blood cell analysis samples,the methods comparison and bias assessment should be conducted periodicallyand the correlation and comparability of the detection results should be evaluated,and the corresponding microscopic review rules to make up for the lack of instrument testing should beestablished,in order to provide accurate,consistent and reliable data for clinical diagnosis and treatment of diseases.

关 键 词：血细胞分析 检测 细胞化学染色 

分 类 号：R446.113[医药卫生—诊断学]