新生期缺氧缺血大鼠脑白质损伤及脑内Fyn、p-ERK和MBP下调  被引量:4

Hypoxic-ischemic white matter injury and down-regulation of Fyn,p-ERK and MBP in neonatal rat brain

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作  者:林凌[1] 张更[1] 邱荣晖[1] 罗道枢[1] 林清[1] Lin Ling;Zhang Geng;Qiu Ronghui;Luo Daoshu;Lin Qing(Department of Human Anatomy, Histology and Embryology, School of Basic Medical Science, Research Center for Neurobiology,Fujian Medical University, Fuzhou 350122, China)

机构地区:[1]福建医科大学基础医学院人体解剖学与组织学胚胎学系,神经生物学研究中心,福州350122

出  处:《中国组织化学与细胞化学杂志》2017年第4期336-341,共6页Chinese Journal of Histochemistry and Cytochemistry

基  金:国家自然科学基金青年科学基金项目(81501305);福建省教育厅重点项目(JA13135)

摘  要:目的检测新生大鼠缺氧缺血后脑内酪氨酸蛋白激酶Fyn、p-ERK及髓鞘碱性蛋白(myelin basic protein,MBP)表达的变化,探讨Fyn-ERK通路在缺氧缺血脑白质损伤中的作用。方法新生3日龄SD大鼠24只,随机分为对照组和缺氧缺血(HI)组。缺氧缺血后4周,应用HE染色法观察脑组织病理学改变;应用免疫荧光染色法和Western blot法观察Fyn、p-ERK及MBP在大鼠脑内的定位定量表达变化。结果 HE染色显示缺氧缺血大鼠出现缺氧缺血脑白质损伤病理改变;免疫荧光染色和Western blot检测显示脑内Fyn、p-ERK和MBP水平均明显下调。结论新生期缺氧缺血损伤4周后,可能通过Fyn与p-ERK水平的下调而使MBP减少,进而使少突胶质细胞成熟障碍,引起脑白质损伤。Objective To understand the role that Fyn-ERK pathway plays in hypoxic-ischemic white matter injury in neonatalrats.Methods3-day-old new born SD rats were randomly divided into control and hypoxic-ischemic groups.The histology of rat braintissues4weeks after operation was examined using HE staining.The distribution and expression level of Fyn,p-ERK and MBP proteinwere detected by immunocytochemistry and western blotting.Results The white matter injury was observed in hypoxic-ischemic rats.The levels of Fyn,p-ERK and MBP protein significantly decreased in the brains of hypoxic-ischemic rats,compared to the controlgroup.Conclusion The results suggest that hypoxia-ischemia may decrease MBP level in neonatal rats through downregulating Fynand p-ERK protein levels,which may lead to oligodendrocyte maturation disorder and white matter injury.

关 键 词:新生期缺氧缺血 Fyn蛋白 髓鞘碱性蛋白 脑白质 

分 类 号:R722.6[医药卫生—儿科]

 

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