新型Gd基T_2造影剂的制备和应用  

A New Gd-Based T_2-Weighted Magnetic Resonance Imaging Contrast Agent: Preparation and Application in Stem Cell Imaging

作　　者：张艳辉[1,2] 张宏岩[2] 张海禄[2] 张朋利[1,2] 蒋海珍[1] 邓宗武[2] 谭波[2] ZHANG Yan-hui;ZHANG Hong-yan;ZHANG Hai-lu;ZHANG Peng-li;JIANG Hai-zhen;DENG Zong-wu;TAN Bo(College of Sciences, Shanghai University, Shanghai 200444, China;Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China)

机构地区：[1]上海大学理学院,上海200444 [2]中国科学院苏州纳米技术与纳米仿生研究所,江苏苏州215123

出　　处：《波谱学杂志》2017年第3期302-310,共9页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(31371010)

摘　　要：设计并合成了结构为TPP-Lys(Acp-DOTA-Gd)-COOH(简称Gd-DOTA-TPP)的小分子磁共振探针,通过电转染的方式用探针标记人源脐带间充质干细胞(hMSCs).11.7 T磁共振成像(MRI)扫描结果表明,Gd-DOTA-TPP标记的hMSCs在细胞内Gd含量为9×10~9 Gd/cell时,T_2加权信号强度即可低至背景信号强度,呈现较强暗信号.将Gd-DOTA-TPP标记的hMSCs移植入小鼠脑室,可明显提高移植干细胞在MRI设备上的检测灵敏度,检测限可低至10~3个细胞.We synthesized TPP-Lys(Acp-DOTA-Gd)-COOH(Gd-DOTA-TPP)as a novel T2-weighted MRI contrast agent,and used it to label human mesenchymal stem cells(hMSCs)via electroporation.hMSCs labeled with Gd-DOTA-TPPpresented a rather dark T2-weighted image at a cellular Gd content of about9?109Gd/cell.The labeled cells weresyringe-injected into a mouse brain with defined cell numbers followed by T2-weighted MRI on an11.7T system,whichyield an in vivo imaging sensitivity of about103cells.

关 键 词：磁共振成像(MRI) 造影剂 人源脐带间充质干细胞(hMSCs) TPP-Lys(Acp-DOTA-Gd)-COOH(Gd-DOTA-TPP) 灵敏度 

分 类 号：O482.53[理学—固体物理]