HPLC法测定心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

Determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Xinshenan granules by HPLC

作　　者：范金钊[1] 丁晓芳[2] 白林[1] 李欣[1] FAN Jin-zhao;DING Xiao-fang;BAI Lin;LI Xin(Department of Preparation Center, PLA General Hospital, Beijing 100853,China;Bengbu Medical College, Bengbu 233000, China)

机构地区：[1]解放军总医院制剂中心,北京100853 [2]安徽蚌埠医学院,安徽蚌埠233000

出　　处：《中国药物应用与监测》2017年第4期209-211,共3页Chinese Journal of Drug Application and Monitoring

摘　　要：目的:建立心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的测定方法。方法:色谱柱:Diamonsil C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(16:84);检测波长:320 nm;流速:1.0 m L·min^(-1);进样量:10μL;柱温:35℃。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在3.767 4~25.116 0μg·m L^(-1)范围内与峰面积呈良好的线性关系(r=1);2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的平均回收率为99.80%;RSD为1.47%(n=9)。结论:本方法操作简单,专属性强,灵敏度高,可用于心肾安颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。Objective:To establish a method for determination of2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside inXinshenan granules.Methods:Diamonsil C18column(250mm×4.6mm,5μm)was used with a mobile phase consisting ofacetonitrile-water(16:84).The detection wavelength was320nm with a flow rate of1.0mL·min-1.Column temperature was35℃.Results:The2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship within the range of3.7674–25.1160μg·mL-1(r=1).The average recovery was99.80%,RSD was1.47%(n=9).Conclusion:This method is simple,accurate andhigh sensitive,which can be used for content determination of2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Xinshenan granules.

关 键 词：HPLC 心肾安颗粒 2 3 5 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 含量测定 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]