IL-2及IL-2Rα基因多态性与汉族儿童支气管哮喘的关联  

Association of IL-2 and IL-2Rα polymorphisms with bronchial asthma in Chinese Han children

作　　者：刘冬松 刘安兵[2] 陈岳明[2] Dong-song Liu;An-bing Liu;Yue-ming Chen(Department of Laboratory Medicine, Jinhua Hospital of Traditional Chinese Medicine,Jinhua, Zhejiang 321017, China;Department of Laboratory Medicine, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, China)

机构地区：[1]浙江省金华市中医医院检验科,浙江金华321017 [2]浙江省杭州市第一人民医院检验科,浙江杭州310006

出　　处：《中国现代医学杂志》2017年第18期38-42,共5页China Journal of Modern Medicine

摘　　要：目的探讨IL-2(rs6822844)及IL-2Rα(rs2104286)基因多态性和汉族儿童支气管哮喘发生的关联。方法随机选取144例支气管哮喘患儿为病例组,228例健康儿童为对照组。用实时荧光定量聚合酶链反应(q RT-PCR)直接测序鉴定各位点基因型;同时采用q RT-PCR检测对照组IL-2Rα各基因型外周血IL-2Rα信使核糖核酸(m RNA)相对表达量,采用酶联免疫吸附试验(ELISA)检测各基因型血浆s IL-2Rα水平。结果IL-2Rα(rs2104286)位点等位基因A在病例组分布频率(86.8%)高于对照组(79.4%)(OR=1.708,95%CI:1.113,2.629,P=0.010),病例组中AA基因型分布频率(75.0%)高于对照组(62.3%)(OR=1.745,95%CI:1.058,2.884,P=0.021);未发现IL-2(rs6822844)位点有多态性分布。对照组中IL-2Rα(rs2104286)位点AA基因型个体外周血IL-2Rαm RNA相对表达量以及血浆s IL-2Rα水平均高于AG基因型(Z=-2.029和-2.361,P=0.043和0.018)。结论 IL-2Rα(rs2104286)基因多态性可能与汉族儿童支气管哮喘发生相关,而且该位点基因型可影响其转录及表达。Objective To investigate the relationship between IL-2(rs6822844)and IL-2Rα(rs2104286)gene Polymorphisms and bronchial asthma in Chinese Han children.Methods A gender and age-matched case control study was conducted.A total of144children with bronchial asthma were enrolled into the case group and228healthy children into the control group randomly.The genotypes were determined by direct sequencing for the products of fluorescent quantitative polymerase chain reaction(PCR).The relative expression of IL-2RαmRNA in the peripheral blood was analyzed by real-time fluorescent PCR.The expression levels of plasma sIL-2Rαwere detected by enzyme-linked immunosorbent assay(ELISA)for the different genotype carriers in the control group.Results The allele A distribution frequency of IL-2Rα(rs2104286)in the case group(86.8%)was significantly higher than that of the control group(79.4%)[OR=1.708(95%CI:1.113,2.629),P=0.010].The AA genotype frequency distribution in the case group(75.0%)was higher than that of the control group(62.3%)[OR=1.745(95%CI:1.058,2.884),P=0.021].The polymorphism distribution of IL-2(rs6822844)was not found in the study.Additionally,both relative expression of IL-2RαmRNA in the peripheral blood and the plasma sIL-2Rαlevel of the individuals with AA genotype were significantly higher than those with AG genotype in the control group(P=0.043and0.018,respectively).Conclusions The gene polymorphism of IL-2Rα(rs2104286)may be associated with the occurrence of bronchial asthma in Chinese Han children,and may regulate gene transcription and expression.

关 键 词：支气管哮喘 基因多态性 白细胞介素2 白细胞介素2a受体 

分 类 号：R562.25[医药卫生—呼吸系统]