补肾中药对肾虚质大鼠生长发育及脑组织胆碱乙酰化酶含量的影响  

Effects of Kidney Reinforcing Medicine on Growth and Development and Brain Choline Acetyl Transferas Content of Rats with Kidney-deficiency Constitution

作　　者：孙瑜嬬[1] 孙理军[1] 孙耀光[1] 张琪[1] 刘熙如 柏云飞 SUN Yu-ru;SUN Li-jun;SUN Yao-guang;ZHANG Qi;LIU Xi-ru;BO Yun-fei(Shaanxi University of Chinese Medicine, Xianyang 712046 China)

机构地区：[1]陕西中医药大学,陕西咸阳712046

出　　处：《中国中医药信息杂志》2017年第9期48-50,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：陕西省重点实验室研究项目(15JS026)

摘　　要：目的探讨补肾中药对肾虚体质大鼠生长发育及脑组织胆碱乙酰化酶(CHAc)含量的调节作用。方法采用"猫吓鼠"方法制作先天不足加后天失养复合型肾虚模型。造模大鼠随机分为模型组、左归丸组和右归丸组,空白组大鼠产自正常孕鼠。对仔鼠恐吓同时开始灌胃给药,药物组给予左归丸和右归丸混悬液,空白组和模型组予等量生理盐水,每日1次,连续3个月。观察大鼠生长发育一般状况,记录5~8周龄各组大鼠体质量和平均食物利用率,ELISA检测大鼠脑组织CHAc含量。结果仔鼠5~8周龄时,模型组较空白组体质量明显减少(P<0.01),左归丸组和右归丸组体质量较模型组明显增加(P<0.05,P<0.01),平均食物利用率与体质量呈正相关;模型组脑组织CHAc含量较空白组明显降低(P<0.01),左归丸组、右归丸组较模型组明显升高(P<0.01)。结论补肾中药可改善肾虚质大鼠生长发育落后状态并调节脑内CHAc含量,从而提高肾虚质大鼠学习记忆能力。Objective To discuss the adjustment effects of kidney reinforcing medicine on growth and development and the content of choline acetyl transferase(CHAc)in rats with kidney-deficiency constitution.Methods The method of“cats scare rats”was used to build composite offspring rat models with deficiency and acquired dystrophy,and then the models were divided into model group,Zuogui Pill group and Yougui Pill group.The rats in blank group came from normal pregnant rats.Baby rats were scared and received gavage at the same time.All administration groups received suspension of Zuigui Pills or Yougui Pills.Blank group and model group received the same amount of normal saline,once a day,for3months.Growth and development of rats were observed.Weight and food utilization of rats aged5-8weeks in each group were recorded.The content of brain CHAc was detected by ELISA.Results When offspring were5-8weeks old,weights of rats in the model group were lower than blank group(P<0.01);while weights of rats in Zuogui Pill group and Yougui Pill group increased significantly(P<0.05)and the food utilization was positively correlated with weight;the CHAc content in the model group decreased significantly compared with blank group(P<0.05,P<0.01);while the CHAc content in Zuogui Pill group and Yougui Pill group increased significantly compared with the model group(P<0.01).Conclusion Kidney reinforcing medicine can improve backward growth and development of rats with kidney-deficiency constitution and adjust CHAc in the brain,so as to promote the learning and memory ability of rats with kidney-deficiency constitution.

关 键 词：补肾中药 生长发育 胆碱乙酰化酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]