芩麻方对慢性阻塞性肺疾病大鼠炎性细胞相关因子的影响  

Effects of Qinma Formula on Inflammatory Cell Related Factors in Rats with Chronic Obstructive Pulmonary Disease

作　　者：丁亚杰[1] 陈晓[2] 俞仲毅[2] 邹纯朴[1] DING Ya-jie;CHEN Xiao;YU Zhong-yi;ZOU Chun-pu(Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Scientific Experimental Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海中医药大学科技实验中心,上海201203

出　　处：《中国中医药信息杂志》2017年第9期55-58,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：全国名老中医药专家传承工作室建设项目(201035);上海进一步加快中医药事业发展三年行动计划(ZY3-CCCX-1-1001)

摘　　要：目的观察芩麻方对慢性阻塞性肺疾病(COPD)大鼠内源性抗炎系统的影响,探讨其作用机制。方法采用改良烟熏联合脂多糖方法制作COPD大鼠模型。将70只SPF级大鼠随机分为正常组、模型组、西药对照组(氨茶碱组)、中药对照组(六君子汤组)和芩麻方低、中、高剂量组,每组10只,各给药组给予相应药物灌胃。光镜下观察大鼠肺组织病理切片;收集大鼠肺泡灌洗液(BALF)和静脉血,对相关指标进行检测;ELISA检测血清白细胞介素(IL)-1β、IL-6、IL-10含量。结果与正常组比较,模型组大鼠BALF和血白细胞、中性粒细胞、巨噬细胞、淋巴细胞、血红蛋白和血小板压积显著增加(P<0.05);与模型组比较,芩麻方中、高剂量组和六君子汤组大鼠BALF白细胞、中性粒细胞、巨噬细胞显著减少(P<0.05),芩麻方中、高剂量组和氨茶碱组大鼠血白细胞、血红蛋白和血小板压积显著减少(P<0.05),血清IL-1β、IL-6含量显著降低(P<0.05),IL-10含量显著升高(P<0.05)。结论芩麻方通过调节炎症反应相关细胞因子从而抑制炎症反应。Objective To investigate the effects of Qinma Formula on endogenous anti-inflammatory system in rats with chronic obstructive pulmonary disease(COPD);To discuss its mechanism of action.Methods Rat models of COPD were made by modified smoked lipopolysaccharide method.70healthy SPF male Wistar rats were randomly divided into normal group,model group,Western medicine control group(aminophylline group),TCM medicine control group(Liujunzi Decoction group)and Qinma Formula high-,medium-,and low-dose groups,with10rats in each group.Each medication group was given relevant medicine for gavage.The pathological changes of lung tissue were observed under light microscope;the alveolar lavage fluid(BALF)and venous blood were collected and the related indexes were tested;the levels of serum IL-1β,IL-6and IL-10were detected by ELISA.Results Compared with normal group,BALF white blood cells,neutrophils,macrophages,lymphocyte,hemoglobin and platelet count in rats of model group increased significantly(P<0.05).Compared with the model group,BALF white blood cells,neutrophils,and macrophages in rats of Qinma medium-and high-dose groups and Liujunzi Decoction group decreased significantly(P<0.05);white blood cells,hemoglobin,and platelet count in rats of Qinma medium-,high-dose groups and aminophylline group decreased significantly(P<0.05);levels of IL-1βand IL-6in serum decreased significantly(P<0.05);level of IL-10in serum increased significantly(P<0.05).Conclusion Qinma Formula can effectively inhibit the inflammatory response through the regulation of inflammatory response-related cytokines.

关 键 词：芩麻方 慢性阻塞性肺疾病 白细胞介素-1Β 白细胞介素-6 白细胞介素-10 大鼠 

分 类 号：R285.5[医药卫生—中药学]