不同产地车前草中总苯乙醇苷和毛蕊花糖苷含量测定  

Content Determination of Phenylethanoid Glycosides and Acteoside in Plantago Herba from Different Producing Areas

作　　者：易满[1] 封传华[1] 汤小林[2] 徐兰[1] 陶晓璇[1] 张浪[1] 李刚[1] YI Man;FENG Chuan-hua;TANG Xiao-lin;XU Lan;TAO Xiao-xuan;ZHANG Lang;LI Gang(No.94 Hospital of PLA, Nanchang 330002, China;Children Hospital of Jiangxi Province, Nanchang 330006, China)

机构地区：[1]解放军第九四医院,江西南昌330002 [2]江西省儿童医院,江西南昌330006

出　　处：《中国中医药信息杂志》2017年第9期84-86,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：2015年度军区医学科技创新课题项目(15MS083)

摘　　要：目的建立车前草中总苯乙醇苷和毛蕊花糖苷的含量测定方法。方法采用紫外-可见分光光度法测定车前草中总苯乙醇苷含量。HPLC测定毛蕊花糖苷含量,色谱柱为ODS2 C18(4.6 mm×150 mm,5μm),流动相为乙腈-0.1%甲酸溶液(13∶87),流速为1 mL/min,检测波长为332 nm,柱温为30℃,进样量为10μL。结果不同产地车前草中总苯乙醇苷含量范围为1.03%~3.47%;毛蕊花糖苷在0.006 2~1.55 mg范围内与峰面积呈良好的线性关系,加样回收率为98.9%(RSD=1.6%),不同产地车前草中毛蕊花糖苷的含量范围为0.18%~0.56%。结论本研究建立的方法操作简单、稳定性好、重复性较好,可用于不同产地车前草中苯乙醇苷类成分及毛蕊花糖苷的含量测定,为车前草的质量控制提供了依据。Objective To establish a method for determination of phenylethanoid glycoside and acteoside in Plantago Herba.Methods UV-visible spectrophotometric method was used for the determination of the content of phenylethanoid glycosides compounds in Plantago Herba.HPLC method was used for the determination of acteoside in Plantago Herba.Chromatographic column with C18ODS2(4.6mm×150mm,5μm)was used.Acetonitrile-0.1%formic acid(13:87)was as mobile phase;the flow rate was1mL/min;the detection wavelength was332nm;the column temperature was30℃;the sample volume was10μL.Results The contents of phenylethanoid glycoside in Plantago Herba from different producing areas were among1.03%-3.47%.Acteoside with peak area over the0.0062-1.55mg range showed a good linear relationship;the sample recovery rate was98.9%,and the RSD was1.6%.The contents of acteoside in Plantago Herba from different producing areas was among0.18%-0.56%.Conclusion The method is simple,stable and reproducible,which can be used for the determination of phenylethanoid glycoside and acteoside in Plantago Herba from different producing areas and provide experimental basis for quality control of Plantago Herba.

关 键 词：车前草 苯乙醇苷 毛蕊花糖苷 

分 类 号：R284.1[医药卫生—中药学]