Feulgen-EA双重染色固定液应用评价  

Application on Feulgen-EA staining fixative

作　　者：黄荣祥 余岚岚 朱晨雁 何文波 马亮 聂琨 张婧 HUANG Rongxiang;YU Lanlan;ZHU Chenyan;HE Wenbo;MA Liang;NIE Kun;ZHANG Jing(Xiamen Motic Medical Laboratory,Xiamen 361006,Fujian,China)

机构地区：[1]厦门麦克奥迪医学检验所,福建厦门361006

出　　处：《检验医学》2017年第8期704-708,共5页Laboratory Medicine

摘　　要：目的对Feulgen-EA染色再固定环节进行应用评价。方法运用细胞图像分析系统对经过冰醋酸(乙酸)、AF、改良AF、Clarke、Bohm-Sprenger 5种固定液再固定后的细胞切片进行扫描,比较细胞核积分光密度(IOD)、变异系数(CV)、Area的差异,获得适合核DNA再固定的固定液,同时探讨温度对固定的影响,通过EA对细胞浆染色,比较胞浆着色效果。结果 DNA再固定宜选用AF或Bohm-Sprenger固定液反应30 min,当温度过低时,影响DNA的固定,相应地延长固定时间,EA对胞浆染色效果良好。结论 AF、Bohm-Sprenger固定液能满足Feulgen-EA双重染色的要求。Objective To investigate Feulgen-EA staining fixative primarily.Methods By image analysis,cells were scanned after fixation by5fixatives[glacial acetic acid(acetic acid),AF,improved AF,Clarke and Bohm-Sprenger],nucleus integrated optical density(IOD),coefficient of variation(CV)and area were compared,and suitable DNA fixative was obtained.The influence of temperature on fixation was evaluated.The effect of cytoplasmic staining was compared by EA staining.Results DNA fixation may choose appropriately AF or Bohm-Sprenger fixed-liquid reaction for30min.When temperature was too low,it may influence on DNA fixation,and the effect of EA to cytoplasmic staining was good.Conclusions AF and Bohm-Sprenger fixatives are suitable for Feulgen-EA double staining.

关 键 词：染色与标记 病理学 图像处理 计算机辅助 固定液 Feulgen-EA染色 

分 类 号：R446.1[医药卫生—诊断学]