中国菜豆凝集素序列的亲缘性分析  

Analysis of Affinity of PHA Sequences of Chinese Phaseolus Vulgaris

作　　者：韩雪[1] 屈晓展 崔勇青 胡子颖 HAN Xue;QU Xiaozhan;CUI Yongqing;HU Ziying(School of Life Sciences,Jianghan University;Hubei Province Engineering Research Center for Legume Plants,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学生命科学学院,湖北省豆类(蔬菜)植物工程技术研究中心,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2017年第4期323-326,共4页Journal of Jianghan University：Natural Science Edition

基　　金：湖北省豆类(蔬菜)植物工程技术研究中心开放性项目(2016-01)

摘　　要：菜豆凝集素可专一与寡糖结合,是其发挥凝血及杀虫功能的主要原因。将菜豆凝集素序列和其他豆科凝集素进行序列对比,发现菜豆凝集素与豇豆凝集素序列相似度最高。对不同国家产地凝集素序列进行对比分析,发现菜豆凝集素高度保守,高度相似氨基酸残基达到74%,中国株菜豆凝集素序列与马来西亚株菜豆凝集素相似度最高,进化树分析显示中国株菜豆凝集素其亲缘性追溯为发源于美洲(美国),经东南亚(马来西亚)传入中国,这与菜豆传入中国的历史相符合。The Phaseolus vulgaris agglutinin(PHA)can be combined with the oligosaccharides specially,which plays an important role on the coagulation and insecticidal function.The leguminous plants lectin sequences were compared,the common bean sequences showed the highest similarity to the cowpea lectin sequences.The common bean lectin sequences in different regions were analyzed by ClustalX2.1and Mega7.0,the similarity in the common bean lectin sequences was about74%and the Chinese common bean lectin showed the high affinity with Malysia common bean lectin.The phylogenetic tree results showed the common bean lectin was originated from America and came into China through Southeast Asia(Malaysia),which is in accordance with the common bean history records.

关 键 词：菜豆 凝集素 序列分析 进化树亲缘性 

分 类 号：S643.1[农业科学—蔬菜学]