地龙抗凝活性物质提取方法研究  

Extraction Method for Anticoagulant Active Substance from Pheretima

作　　者：杨新[1] 刘欣[1] 涂乾[1] 万明[1] 张涛[1] YANG Xin;LIU Xin;TU Qian;WAN Ming;ZHANG Tao(Department of Pharmacy,School of Medicine,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学医学院药学系,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2017年第4期343-347,共5页Journal of Jianghan University：Natural Science Edition

基　　金：湖北省教育厅科研项目(B2013163)

摘　　要：目的比较不同提取方法对两种不同品种的地龙——广地龙和沪地龙提取物抗凝活性的影响,分别确定两种地龙的最佳提取方法。方法应用凝血酶滴定法分别比较两个不同品种地龙干品水煎煮、0.9% NaCl煎煮、70%乙醇回流提取、石油醚脱脂后水煎煮、石油醚脱脂后0.9% NaCl煎煮及石油醚脱脂后70%乙醇回流提取6种不同提取方法提取物中抗凝活性物质的差异。结果以宽体金钱蛭为阳性对照,与空白组、阴性组对照后,两种地龙分别在6种提取方法下提取物均表现抗凝活性,并且两种地龙均是石油醚脱脂后0.9% NaCl煎煮提取物中抗凝活性最强。结论不同品种的地龙抗凝活性强度与提取前处理、提取方法有密切联系,干品石油醚脱脂有利于地龙抗凝活性物质的提取。Objective To compare the different extraction methods for anticoagulant active substances from two kinds of Pheretima to obtain the best method.Methods Six methods were applied to extract anticoagulant activity substance from two kinds of Pheretima,including water decocting method,0.9%NaCl solution decocting method,70%ethanol reflux extraction,water decocting after degreasing with petroleum ether,0.9%NaCl solution decocting after degreasing with petroleum ether,70%ethanol reflux extraction after degreasing with petroleum ether,and detected the activity of the substance with thrombin titration method.Results With the Whitmania pigra as positive control group,after being compared with blank and negative control group,the results showed the extracts from two kinds of Pheretima expressed anticoagulant activity under the six extraction methods.Among these six methods,the anticoagulant activity extracted with0.9%NaCl solution decocting after degreasing with petroleum ether was strongest.Conclusion The anticoagulant activity of extracts from different kinds of Pheretima are associated with pre-treatment and extraction methods.The degreasing with petroleum ether is in favor of the extraction.

关 键 词：不同品种的地龙干品 提取方法 抗凝活性 凝血酶滴定法 

分 类 号：R973.2[医药卫生—药品]