油菜素内酯对盐胁迫下香樟幼苗叶片抗氧化酶活性的影响  

Effects of exogenous 2,4-epibrassinolide on antioxidant enzyme activities of camphor seedlings under salt stress

作　　者：王舒甜 王金平[1] 张金池[1] 岳健敏 WANG Shutian;WANG Jinping;ZHANG Jinchi;YUE Jianmin(Key Laboratory of Soil and Water Conservation and Ecological Restoration in Jiangsu Province/Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]南京林业大学,江苏省南方现代林业协同创新中心/江苏省水土保持与生态修复重点实验室,南京210037

出　　处：《浙江大学学报（农业与生命科学版）》2017年第4期476-482,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：江苏省高校优势学科建设工程项目(PAPD);江苏省林业三新工程项目(LYSX[2014]05)

摘　　要：为揭示油菜素内酯(2,4-epibrassinolide,EBR)对香樟耐盐性的影响,以香樟为试验材料,在4‰单一盐胁迫下,采用EBR浸种和浸种+喷叶2种处理方法,分别施加不同质量浓度EBR(0.1、0.2、0.3、0.4、0.5 mg/L),以蒸馏水处理为对照,比较分析盐胁迫下香樟幼苗的抗氧化酶活性变化。结果表明:在4‰盐胁迫下,植株叶片中丙二醛、可溶性糖和可溶性蛋白质含量及抗氧化酶活性均增加;施用一定质量浓度的EBR能提高叶片超氧化物歧化酶、过氧化物酶、过氧化氢酶等保护酶的活性,增加可溶性蛋白质及可溶性糖含量,并降低丙二醛含量,且浸种+喷叶处理优于单一浸种处理。综上所述,在盐胁迫下适宜质量浓度的EBR可提高叶片抗氧化能力,清除叶片中的活性氧,保护膜结构,缓解盐胁迫对香樟的伤害,从而提高香樟的抗盐性;在0.1~0.5 mg/L EBR质量浓度梯度中,以0.4和0.5 mg/L的效果较好,但对于EBR最佳质量浓度的确定还有待进一步研究。Cinnamomum camphora,also known as camphor tree,is a kind of excellent tree for afforestation,street and shade,which is of great use to water conservation,making environment green and beauty.Cinnamomum camphora prefers warm and humid climate,and has strong taproot,deep-rootedness and strong sprouting ability,which is of considerable application value in southern coastal areas of China.However,camphor is intolerant to drought,barren and saline soil,and its requirement for soil salinity should be within0.2%.Camphor grows well in acid yellow soil,but is susceptible to yellow leaf disease in alkaline soil.Moreover,some other elements such as low temperature,weak light and soil secondary salt damage of high concentration would lead to growth failure,fertility inhibition,and even death of camphor.In coastal areas of Jiangsu Province,high soil pH and low organic matter content are not beneficial to the growth and development of camphor,which seriously affect the greening cultivation,introduction and popularization.Therefore,it is of great significance to study the mechanism of salinity tolerance of C.camphora.

关 键 词：香樟幼苗 油菜素内酯 盐胁迫 抗氧化酶活性 活性氧 

分 类 号：S718.3[农业科学—林学]