1997-2010年上海市猪源大肠杆菌耐药性和产ESBLs菌的基因型检测  

Drug Resistance of Escherichia coli Isolated from Swine in Shanghai City from 1997 to 2010 and Genotype Detection of Strains Expressing ESBLs

作　　者：沈莉萍[1] 徐锋[1] 张维谊[1] 王建[1] Shen Liping;Xu Feng;Zhang Weiyi;Wang Jian(Shanghai Animal Disease Prevention and Control Center,Shanghai　201103)

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《中国动物检疫》2017年第9期26-30,45,共6页China Animal Health Inspection

基　　金：上海市农业基础性项目(沪农科攻字[2014﹞第7-3-3号)

摘　　要：[目的 ]了解1997—2010年上海市猪源大肠杆菌的耐药情况和产ESBLs大肠杆菌的基因型分布。[方法 ]采用K-B法对199株猪源大肠杆菌进行药敏试验,采用PCR方法对TEM、CTX-M、SHV和OXA等4种基因型进行检测。[结果 ]199株猪源大肠杆菌对20种抗菌药物均表现出不同程度的耐药性。耐药率最低的为大观霉素(7.04%),其次为头孢曲松(10.55%)、头孢哌酮(12.56%),最高的为林可霉素(100%),对阿莫西林和复方新诺明的耐药率分别高于80%和93.33%。5~15耐的菌株占88.94%。15种抗菌药物之间均存在不同程度交叉耐药性。共检出15株产ESBLs菌株(7.54%),其中2株TEM型,5株CTX-M型,7株同时携带TEM、CTX-M型。[结论 ]上海市猪源大肠杆菌分离株耐药谱广、耐药率高;对抗菌药物的交叉耐药现象严重,交叉耐药率有的相似,有的差异大。猪源大肠杆菌ESBLs基因型主要以TEM型和CTX-M型为主,尚无SHV型和OXA型。[Objective]To investigate the drug resistance of E.coli from swine in Shanghai from1997to2010and genotype distribution of strains expressing ESBLs.[Methods]The antimicrobial susceptibility of199strains of E.coli isolated from swine was determined by K-B method,and the genotypes of TEM,CTX-M,SHV and OXA genes were analyzed by PCR.[Results]199strains of E.coli isolated from swine showed different degrees of resistance to20kinds of antibiotics.The lowest rate of drug resistance was spectinomycin(7.04%),followed by ceftriaxone(10.55%)and cefoperazone(12.56%),the highest was lincomycin(100%),and the resistance rates to amoxicillin and compound sulfamethoxazole were greater than80%and93.33%,respectively.88.94%of the isolates exhibited multiple resistance from5to15.There was cross-resistance among the15kinds of antibiotics.There were15strains expressing ESBLs and the detection rate was7.54%,including2strains expressing TEM gene,5strains expressing CTX-M gene,and7strains expressing both TEM gene and CTX-M gene.[Conclusion]The isolates of E.coli from swine in Shanghai had wide spectrum of resistance and high resistance rates.Cross-resistance of antibiotics was serious.Cross-resistance rate was somewhat similar,but some were different.TEM type and CTX-M type were predominant genotypes of ESBLs in E.coli strains,and both SHV type and OXA weren′t detected.

关 键 词：猪 大肠杆菌 耐药性 ESBLS 基因型 上海 

分 类 号：S852.612[农业科学—基础兽医学]