池蝶蚌β-连环蛋白基因cDNA的克隆及表达特征分析  

THE STRUCTURAL FEATURE AND EXPRESSION ANALISIS OF HSβ-CATENIN cDNA AND PROTEIN FROM HYRIOPSIS SCHLEGELII

作　　者：闫含笑 史建伍[1] 盛军庆[1] 王军花[1] 洪一江[1] YAN Han-Xiao;SHI Jian-Wu;SHENG Jun-Qing;WANG Jun-Hua;Hong Yi-Jiang(The College of Life Science, Nanchang University, Nanchang 330000, China)

机构地区：[1]南昌大学生命科学学院,南昌330000

出　　处：《水生生物学报》2017年第5期972-976,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31660337);江西省教育厅项目(GJJ150166)资助~~

摘　　要：为了解淡水贝类性别调控与分化机制,课题组建立了池蝶蚌(Hyriopsis schlegelii)性腺转录组,在转录组库中,存在β-连环蛋白(β-catenin)基因序列。实验对池蝶蚌β-catenin基因进行验证,采用RACE技术克隆其c DNA全长,命名为Hsβ-catenin。该序列全长4386 bp,5′-非编码区为162 bp,3′-非编码区为1758 bp,开放阅读框为2466 bp,编码821个氨基酸;该蛋白结构域主要由12个ARM重复序列组成;二级结构中,α-螺旋占47.75%,β-折叠占1.22%,随机卷曲占51.04%;三级结构中含大量α-螺旋且为右手超螺旋,构成ARM结构域;系统进化树分析表明,Hsβ-catenin与软体动物聚为一支,然后与昆虫类聚为一支。实时荧光定量PCR(q RT-PCR)检测显示,Hsβ-catenin在肠中表达量最高,其次是斧足和精巢。Hsβ-catenin基因在12月龄和36月龄表达量较高,且在36月龄表达量最高,表明其可能参与池蝶蚌的性别调控与分化作用。In order to explore the mechanism of sexual development of freshwater shellfish,we established a gonadaltranscriptome of Hyriopsis schlegelii.Based on the transcriptome library,this study investigated theβ-catenin.The fulllengthcDNA was cloned by RACE PCR,which named Hsβ-catenin.The full length of Hsβ-catenin cDNA was4386bpthat contained an open reading frame(ORF)of2466bp coding a protein of821amino acids.The protein was mainlycomposed of12ARM repeat sequences.In the secondary structure,theα-helix accounted for47.75%and theβ-foldingaccounted for1.22%were predicted.The tertiary structure had manyα-helixs,namely ARM domain.Hsβ-catenin genewas clustered with homologous genes from soft animal in the phylogenetic tree.Hsβ-catenin expressed highly in intestineand modestlyin foot and testis.The expression level of Hsβ-catenin was high at the age of12-month-old(genderdifferentiation)and36-month-old(gonad mature),indicated that it might be involved in sexual development.

关 键 词：池蝶蚌 Hsβ-catenin 组织表达 克隆 序列分析 QRT-PCR 

分 类 号：Q3441[生物学—遗传学]