大亚湾水域并基拟菱形藻的种类鉴定和产毒特征分析  

THE IDENTIFICATION OF PSEUDO-NITZSCHIA DECIPIENS FROM DAYA BAY AND ANALYSIS OF ITS DOMOIC ACID PRODUCTION

作　　者：黄春秀[1] 徐国双[1] 李扬[1] HUANG Chun-Xiu;XU Guo-Shuang;LI Yang(Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, College of Life Science, South China Normal University, Guangzhou 510631, China)

机构地区：[1]华南师范大学生命科学学院,广州市亚热带生物多样性与环境生物监测重点实验室,广州510631

出　　处：《水生生物学报》2017年第5期1118-1125,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31570205,31370235);广东省科技计划项目(2015A030401084)资助

摘　　要：为了丰富我国海域拟菱形藻(Pseudo-nitzschia Peragallo)的物种多样性,并澄清其产毒特征,研究从广东大亚湾海域分离并建立了一株拟菱形藻的单克隆培养株系MC298,通过光学显微镜下的群体特征和透射电镜下的超微形态特征观察,结合基于核糖体转录间隔区(Internal Transcribed Spacer,ITS)的分子系统学数据,以及基于ITS2转录RNA的二级结构分析,鉴定到我国拟菱形藻属的1个新记录种:并基拟菱形藻P.decipiens Lundholm&Moestrup。研究对其形态学特征进行了较为详细地描述,并与相似种进行了比较,还对其ITS2-RNA的特有标志结构进行了阐述。同时,利用高效液相色谱-质谱联用法(Liquid chromatography tandem mass spectrometry,LC-MS/MS)对该藻株的产毒特征进行了检测,结果未检测到DA的存在。研究不仅丰富了我国拟菱形藻属的物种多样性,也可为拟菱形藻的产毒特征研究提供基础数据。The marine diatom genus Pseudo-nitzschia Peragallo,the main biological source of Amnesic Shellfish Poisoning(ASP)-domoic acid(DA),has attracted great attention worldwide,and has became the hotspot in the researchfield of phycology and environmental toxins.Lots of progress has been made in the past two decades with48newly describedtaxa and24species that can produce DA.In China,a total of17taxa has been reported without any detectableDA from Chinese Pseudo-nitzschia strains.To investigate the species diversity of Pseudo-nitzschia in Chinese coastand the ability of DA production for Chinese Pseudo-nitzschia,monoclonal strains were isolated and established fromDaya Bay,the South China Sea.These strains were identified based on the morphological characteristics by light microscopyand transmission electron microscopy and molecular analysis using the internal transcribed spacer region ofribosomal rRNA encoding gene,and the secondary structure of ITS2RNA transcripts.We reported one newly recordedspecies for China,P.decipiens Lundholm&Moestrup.Its morphology was described and compared with bothmorphology-similar and molecule-close taxa,and the unique molecular feature of the secondary structure of ITS2-RNAwas analyzed.No detectable DA was observed from this species using a single liquid chromatography tandem massspectrometry(LC-MS/MS)injection.This study enriches the diversity of the genus Pseudo-nitzschia in China,and mayprovide detail information for the further studies about DA production of Chinese Pseudo-nitzschia strains.

关 键 词：并基拟菱形藻 分类学 形态 核糖体转录间隔区 ITS2-RNA二级结构 多莫酸 

分 类 号：Q949.27[生物学—植物学]