一种快速提取南瓜大群体基因组DNA的方法  

A Rapid DNA Extraction Method for Large Squash Population

作　　者：李委[1,2] 戴祖云 夏伟伟 王雯雯 LI Wei;DAI Zu-yun;XIA Wei-wei;WANG Wen-wen(Anhui Jianghuai Horticulture Seed Co. Ltd.,Hefei 230031,Anhui,China;College of Horticulture,Anhui Agricultural University,Hefei 230061,Anhui,China)

机构地区：[1]安徽江淮园艺种业股份有限公司,安徽合肥230031 [2]安徽农业大学园艺学院,安徽合肥230061

出　　处：《中国蔬菜》2017年第9期36-40,共5页China Vegetables

基　　金：安徽省科技攻关计划项目(1501142220)

摘　　要：基因型鉴定是作物分子标记辅助育种和基因功能遗传分析中十分重要的环节,此项技术的应用正日渐频繁,因而迫切需要一种高通量筛选的方法。本试验以南瓜中筛选到的互补型SSR引物为验证手段,在种子阶段比较了钻孔法和萌芽法采用碱煮法提取DNA的效果,并且在成熟植株阶段验证了各组织部位采用碱煮法提取DNA的效果。结果表明:使用萌芽的南瓜种子根尖以碱煮法提取DNA是可用于种子纯度测定的快速方法;成熟植株各部位采用碱煮法提取DNA均可用于植株基因型筛选。Genotype identification is an important link in molecular-marker-assisted breeding and geneticanalysis of gene function.Since this method is used more and more frequently,researchers need a high-throughputtechnique.This experiment took a pair of SSR primers of complementary type as the method,and comparedthe effect of extracting DNA from cotyledon and root during seedling stage.The effect of extracting DNA from eachtissue of the plant by NaOH-boiling method during maturing stage was also verified.The results indicated thatextracting DNA from root with NaOH-boiling method was a rapid way to measure seed purity.Therefore,extractingDNA with NaOH-boiling method from all tissues of the matured plant could be used for screening plant genotype.

关 键 词：南瓜 基因型鉴定 分子育种 碱煮法 

分 类 号：S642.1[农业科学—蔬菜学]