隔山消对豚鼠离体回肠收缩的促进作用及机制探讨  

Exploring the Contractile Effect of Geranium strictipes R. Knuth on the Intestinal Muscle of the Isolated Guinea-pig Ileum and Its Mechanism

作　　者：耿玲 李洪文 陈俊雅 Geng Ling;Li Hongwen;Chen Junya(Pre-clinical College, Dali University, Dali, Yunnan 671000, China;Chuxiong Pharmaceutical College, Chuxiong,Yunnan 675000, China;College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]楚雄医药高等专科学校,云南楚雄675000 [3]大理大学药学与化学学院,云南大理671000

出　　处：《大理大学学报》2017年第8期10-12,共3页Journal of Dali University

基　　金：大理大学青年教师科研基金资助项目(KYQN201309)

摘　　要：目的:观察隔山消乙酸乙酯提取物对离体豚鼠回肠平滑肌收缩活动的影响,并初步探讨其体外作用机制。方法:制备豚鼠离体回肠标本,观察平滑肌正常收缩曲线,分别记录单独使用不同浓度隔山消和使用阻断剂后隔山消对回肠平滑肌的收缩作用。结果:不同浓度隔山消对离体豚鼠回肠平滑肌收缩促进作用明显,与空白对照组比较,20、40 mg/mL引起平滑肌收缩的最大张力、频率和幅度差异具有统计学意义(P<0.01),且其可有效对抗阿托品、氯苯那敏导致的豚鼠回肠舒张作用。结论:隔山消对豚鼠离体回肠平滑肌的收缩活动有兴奋作用,其引起的收缩效应可能与M受体和H_1受体有关。Objective:To observe the effect of ethyl acetate extracts from Geranium strictipes R.Knuth on the isolated guinea-pigileum smooth muscle,and to explore the mechanism of its in vitro action.Methods:The normal contraction curve was observed afterthe isolated ileum specimens were prepared.Then contraction in smooth muscle caused by Geranium strictipes R.Knuth and threeblockers was recorded.Results:The Geranium strictipes R.Knuth in different doses could accelerate the ileum smooth musclecontraction of the guinea-pig.The maximum tension,frequency and amplitude induced by20and40mg/mL extracts had a statisticaldifference(P<0.01)compared with the normal group;and it could resist against ileum smooth muscle relaxation induced by Atropineand Chlorobenzene.Conclusion:The Geranium strictipes R.Knuth has stimulating effects on ileum smooth muscle extraction of theguinea-pig,and the contractile effect may be related to M receptor and H1receptor.

关 键 词：隔山消 离体回肠 M受体 H1受体 

分 类 号：R284.3[医药卫生—中药学]