葡萄砧木的耐盐性评价  

Evaluation of grape rootstock salt tolerance

作　　者：吴梦晓[1] 张晓雪 李众 王雅楠 张剑侠[1] WU Mengxiao;ZHANG Xiaoxue;LI Zhong;WANG Ya-nan;ZHANG Jianxia(College of Horticulture, Northwest A & F University / State Key Laboratory of Crop Stress Biology in Arid Areas,Northwest A & F University / Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture, Yangling, Shaanxi 712100)

机构地区：[1]西北农林科技大学园艺学院/旱区作物逆境生物学国家重点实验室/农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌712100

出　　处：《中外葡萄与葡萄酒》2017年第5期14-18,共5页Sino-Overseas Grapevine & Wine

基　　金：国家科技支撑计划子课题(2013BAD02B04-06);国家级大学生创新创业训练计划(201510712009)

摘　　要：以20份葡萄砧木材料及2个对照栽培品种玫瑰香、红地球的半木质化单芽茎段为外植体建立无菌系,然后在1/2 MS+IBA 0.2 mg/L+NAA 0.05 mg/L继代培养基上获得组培苗,取组培苗单芽小茎段接种于添加不同质量浓度NaCl的继代培养基上进行耐盐性筛选。结果表明:盐胁迫50 d后,组培苗的盐害指数随盐浓度的提高而增加,株高、根长和一级根数目均随盐浓度的提高而降低。以盐害指数作为主指标,参考株高、根长和一级根数目3项指标,供试葡萄材料的耐盐性分为4个类型:红地球、玫瑰香、河山-1、Dog Ridge、6-12-6、00-1-10属于耐盐类型;YH6、YH56、00-1-5、左山75097、1-1-8属于较耐盐类型;188-08、101-14、河岸葡萄(♀)、1-1-6、SO4、6-12-1、YT2、6-12-4、YT48属于盐较敏感类型;燕山-1、1103P属于盐敏感类型。The sterile system of explants of20grape rootstock materials and two control Vitis vinifera cv Muscat Hamburg(CK1)and Red Globe(CK2)were established using semi-lignified single-bud stem;and then the tube plantlets were obtained by subculture in1/2MS+IBA0.2mg/L+0.05mg/L NAA medium;finally,salt tolerance experiment of grape tube plantlets were carried out by adding different concentration of NaCl in subculture medium.The results showed that:fifty days later,salt injury index of tube plantlets increased with the increase of salt concentration,plant height,root length and root number were reduced with the increase of salt concentration.With the salt injury index as the main index,and three indicators plant height,root length and root number as reference,salt tolerance of the tested grape materials could be divided into four types:Red Globe,Muscat Hamburg,Heshan-1,Dog Ridge,6-12-6and00-1-10were salt tolerance type;YH6,YH56,00-1-5,Zuoshan75097,1-1-8were slight salt tolerance type;188-08,101-14,Vitis riparia(♀),1-1-6,SO4,6-12-1,YT2,6-12-1and YT48were slight salt sensitive type;Yanshan-1and1103P were salt sensitive type.

关 键 词：葡萄 砧木 组织培养 试管苗 耐盐性 评价 

分 类 号：S663.1[农业科学—果树学] S603.4[农业科学—园艺学]