褐飞虱热激蛋白70在不同温度胁迫下的差异表达特性研究  

Induced Expression Profiles of Hsp70s in Brown Planthoppers, Nilaparvata lugens, Under Different Temperatures

作　　者：单丹 #王利华[2] #张月亮[2] 韩阳春[1] 牛洪涛 潘磊[2] 方继朝 王利华[2] 张月亮 SHAN Dan;WANG Lihua;ZHANG Yueliang;HAN Yangchun;NIU Hongtao;PAN Lei;FANG Jichao;WANG Lihua;ZHANG Yueliang(College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

机构地区：[1]南京农业大学植物保护学院,南京210095 [2]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国水稻科学》2017年第5期533-541,共9页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31572004;31301660);江苏省自然科学基金资助项目(BK20130711);江苏省农业自主创新资金资助项目(6111608)

摘　　要：【目的】褐飞虱是我国主要水稻害虫之一,随着全球气候变暖,其越冬范围逐年扩大。为了明确褐飞虱对温度的适应机制,研究了其热激蛋白70基因家族的组成及温度诱导表达特性。【方法】利用转录组测序和Gen Bank数据库获取热激蛋白70基因序列,采用MEGA4.1邻接法构建进化树,利用实时荧光定量PCR研究其温度诱导表达特性。【结果】筛选出与热激蛋白70基因同源性较高且具有完整开放阅读框的序列共15个,其中8个在正常或温度胁迫下表达,可能分布在细胞质/细胞核、内质网、线粒体等器官,且本身表达丰度存在显著差异。30~44℃高温胁迫后KX976471、KX976473、KX976475、KX976476、KX976477和KX976478等6个基因的表达量不同程度上调,最大上调倍数为1.72~245.33;0~22℃低温胁迫后,除KX976475表达量上调2.38倍外,其余基因表达量变化不显著或下降。【结论】褐飞虱8个热激蛋白70基因在正常或温度胁迫下表达,可能分布于细胞质/核、线粒体、内质网;在其高温适应性中可能起重要作用,但在低温适应性中的作用有限。【Objective】The brown planthopper(BPH),Nilaparvata lugens,is one of the major insect pests on rice in China.In recent years,the overwintering area of BPH expanded with global warming.In order to explore the response mechanisms of BPH to temperature,the hsp70s were cloned and the induced expression profiles of these genes were analyzed.【Method】The nucleotide sequences of hsp70s were gotten from transcriptome sequencing and GenBank database,the phylogenetic tree of HSP70was based on neighbor-joining method by MEGA4.1,and the induced expression profiles were analyzed by real-time quantitative PCR.【Result】It was found that fifteen hsp70homologous genes with complete open reading frame were gotten in BPH,and8out of15genes were expressed in normal conditions or temperature stress.These eight hsp70s were distributed in the cytoplasm/nucleus,endoplasmic reticulum ormitochondria,and had significantly different expression in normal conditions.After30-44°C high temperature treatment,the expression levels of KX976471,KX976473,KX976475,KX976476,KX976477and KX976478were up-regulated,and the maximum increased times was1.72-245.33.However,only the expression level of KX976475increased by2.38times,the other seven were not changed or even significantly decreased in expression level after0-22°C temperature treatment.【Conclusion】It was found that eight hsp70s were expressed in normal conditions or temperature stress in the cytoplasm/nucleus,endoplasmic reticulum or mitochondria.These hsp70s might play an important role in high temperature adaptation,but a limited role in cold adaptation in BPH.

关 键 词：褐飞虱 温度 热激蛋白70 诱导表达 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]