水稻对穗枯病的抗病机理初步研究  

A Primary Study on the Mechanism Behind Resistance to Bacterial Panicle Blight of Rice

作　　者：李路[1,2] 徐以华 梁梦琦 王玲[2] 刘连盟[2] 侯雨萱 黎起秦[1] 黄世文[1,2] LI Lu;XU Yihua;LIANG Mengqi;WANG Ling;LIU Lianmeng;HOU Yuxuan;LI Qiqin;HUANG Shiwen(College of Agronomy, Guangxi University, Nanning 530003, China;China National Rice Research Institute, Hangzhou 310006,China)

机构地区：[1]广西大学农学院,南宁530003 [2]中国水稻研究所,杭州310006

出　　处：《中国水稻科学》2017年第5期551-558,共8页Chinese Journal of Rice Science

基　　金：国家重点研发计划资助项目(2016YFD0200801);中国农业科学院科技创新工程资助项目(CAAS-ASTIP-2013-CNRRI);中央级公益性科研院所基本科研业务费专项(2014RG005-2);浙江省三农六方项目(CTZB-F160728AWZ-SNY1-4)

摘　　要：【目的】水稻在孕穗期比苗期更容易感染穗枯病(Bacterial panicle blight of rice)并出现病症。本研究旨在探究水稻不同时期对穗枯病的抗性机理,为培育抗病水稻品种奠定基础。【方法】采用喷雾法和注射法分别对苗期和孕穗期的抗、感病水稻品种接种颖壳伯克氏菌(Burkholderia glumae),测定处理组与对照组的3种抗氧化酶(POD、CAT、SOD)活性的差异,并利用实时荧光定量PCR测定5种防卫反应基因(PR1a、PR10b、Rcht、LOX、PAL)的表达量。【结果】B.glumae的侵染能引起水稻活性氧的积累,提高抗氧化酶活性,使部分防卫反应基因大量表达,但是苗期和孕穗期的应答有较大区别。水稻孕穗期的抗氧化酶(SOD、CAT和POD)活性和防卫反应基因(PR10b、Rcht和PAL)的表达量比苗期高,但PR1a和LOX的表达量却低于苗期。【结论】B.glumae能诱导孕穗期的水稻产生更多抗病反应,参与抗病反应的主要是水杨酸信号传导途径。【Objective】Rice is more susceptible to bacterial panicle blight at the booting stage than seedling stage.Theobjective of the study is to understand the resistant mechanism in different growing stage of rice and lay a basis forbreeding rice varieties with high resistance【.Method】The spraying and injection method were used to inoculate seedlingsand panicles of resistant and susceptible rice with Burkholderia glumae,respectively.The activities of three antioxidantenzymes(peroxidase,catalase and superoxide dismutase)of treatments and control were investigated.Real-time PCRwas used to detect the expression of five defense response genes(PR1a,PR10b,Rcht,LOX and PAL).【Result】Theinfection of B.glumae induced the accumulation of reactive oxygen species,the increase of antioxidant enzymesactivities and the higher expression of some defense genes.However,the defense response was different at the seedlingstage and booting stage of rice.At the booting stage,the activities of antioxidant enzymes(peroxidase,catalase andsuperoxide dismutase)and the expression of PR10b,Rcht and PAL were higher,but the expression levels of PR1a andLOX were lower than those at the seedling stage.【Conclusion】B.glumae could induce more defense response of rice inbooting stage,salicylic acid signaling pathway played an important role during the defense.

关 键 词：水稻 穗枯病 抗氧化酶 防卫反应基因 实时荧光定量PCR 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]