糖痛方对高糖诱导RSC96细胞凋亡与增殖的影响  

Effects of Tangtong Formula on RSC96 Schwann Cells Apoptosis and Proliferation Induced by High Glucose

作　　者：刘晓星 朱晓云[1] 韦茂英 邓岚[1] 李鸣镝[1] LIU Xiao-xing;ZHU Xiao-yun;WEI Mao-ying;DENG Lan;LI Ming-di(Department of Endocrinology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China)

机构地区：[1]中国中医科学院广安门医院内分泌科,北京100053

出　　处：《中国中医药信息杂志》2017年第10期49-52,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81473464)

摘　　要：目的筛选中药糖痛方的体外作用最佳剂量。方法采用含5~125 mmol/L不同浓度葡萄糖的DMEM培养基培养RSC96细胞,检测不同浓度(0.25、0.5、0.75、1.0、1.5、2.0、3.0、5.0 mg/m L)糖痛方高糖培养RSC96细胞增殖。采用含100、125 mmol/L葡萄糖培养基培养RSC96细胞72 h后,AV/PI凋亡试剂盒和流式细胞仪检测细胞凋亡,计算细胞凋亡率;CCK-8法检测不同时点RSC96细胞增殖。结果 RSC96细胞在100、125 mmol/L葡萄糖培养基培养下发生凋亡,凋亡率分别为(7.46±0.96)%、(16.53±1.01)%,与对照组比较差异有统计学意义(P<0.05);不同浓度糖痛方可明显改善高糖对RSC96细胞增殖的抑制作用,24 h起效浓度为0.25 mg/m L;0.5、1.0、1.5 mg/m L 3个剂量糖痛方可明显抑制高糖诱导的细胞凋亡,与125 mmol/L高糖组比较,细胞凋亡率显著降低(P<0.05,P<0.01)。结论在3个不同剂量中,1.5 mg/m L糖痛方抑制细胞凋亡作用最佳。Objective To screen the optimal dose of Tangtong Formula in vitro.Methods RSC96Schwann Cells were cultivate by DMEM mediums which contains different concentrations of glucose(5-125mmol/L).The prevention effects of Tangtong Formula at different concentrations(0.25,0.5,0.75,1.0,1.5,2.0,3.0,5.0mg/mL)on the proliferation of RSC96Schwann Cells induced by high glucose were detected.After the RSC96Schwann Cells were cultivated in100mmol/L and125mmol/L high glucose mediums for72h,the apoptosis of RSC96Schwann Cells was detected by flow cytometry Annexin V/PI,and the apoptosis rate was calculate;the proliferation situation of RSC96Schwann cells in different times was detected by CCK-8method.Results RSC96Schwann cells were in after being intervened by100mmol/L and125mmol/L high glucose mediums.The apoptosis rates were respectively(7.46±0.96)%and(16.53±1.01)%,with statistical significance compared with control group(P<0.01).Different concentrations of Tangtong Formula could alleviate the inhibitory effect of high glucose on the proliferation of RSC96Schwann cells,and the threshold concentration of Tangtong Formula in24h was0.25mg/mL.The concentrations of Tangtong Formula in0.5mg/mL,1.0mg/mL,and1.5mg/mL could inhibit the apoptosis of RSC96Schwann cells induced by high glucos,compared with125mmol/L high glucose group,the apoptosis rate of RSC96Schwann cells decreased significantly(P<0.05,P<0.01).Conclusion Among three different doses,when the dose of Tangtong Formula is in1.5mg/mL,the effects on inhibiting apoptosis are the best.

关 键 词：糖痛方 RSC96细胞 细胞凋亡 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]