RF对ELISA检测血清HBeAg假阴性干扰的分析  

False negative interference of RF on serum HBeAg by ELISA

作　　者：谢良才[1] 范文[1] 徐磊 许蓉[1] 刘天春[1] XIE Liangcai;FAN Wen;XU Lei;XU Rong;LIU Tianchun(Department of Clinical Laboratory,Jingzhou First People's Hospital,Jingzhou 434000,Hubei,China;Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,Hubei,China)

机构地区：[1]荆州市第一人民医院检验科,湖北荆州434000 [2]华中科技大学同济医学院附属协和医院,湖北武汉430000

出　　处：《检验医学》2017年第9期765-768,共4页Laboratory Medicine

摘　　要：目的证实类风湿因子(RF)对酶联免疫吸附试验(ELISA)检测乙型肝炎e抗原(HBe Ag)假阴性干扰的存在。方法收集乙型肝炎表面抗原(HBs Ag)、HBe Ag均阴性而RF阳性新鲜血清样本,与HBe Ag阳性对照品9∶1混合配成HBe Ag阳性且RF阳性的试验样本,分别用不同的ELISA试剂检测样本的HBe Ag。使用RF吸附乳胶对干扰样本进行预处理,比较预处理前后HBe Ag水平的变化。结果试验中RF对3种不同厂商生产的HBe Ag ELISA试剂均有不同程度的假阴性干扰。9例假阴性样本预处理后有7例转为阳性。结论 RF可影响ELISA检测HBe Ag的S/CO值,引起假阴性结果,样本预处理可以有效消除大部分RF的干扰。Objective To investigate whether rheumatoid factor(RF)can cause false negative interferenceon serum hepatitis B e antigen(HBeAg)by enzyme-linked immunosorbent assay(ELISA).Methods Serumsamples with negative hepatitis B surface antigen(HBsAg),negative HBeAg and positive RF were collectedand comprised by blending HBeAg-positive and RF-positive control substance at a ratio of9∶1.HBeAg level wasdetermined by different ELISA kits.RF adsorption latex was used to remove RF from samples,and the changeof HBeAg level was compared before and after pre-treatment.Results The3kinds of HBeAg ELISA kits hadfalse negative interference.There were7of9false negative samples transforming to positive after pre-treatment.Conclusions The S/CO of HBeAg determination by ELISA is susceptible to false negative interference from RF.Thepre-treatment of samples is effective prior to the interpretation of determination results.

关 键 词：类风湿因子 干扰 酶联免疫吸附试验 乙型肝炎E抗原 

分 类 号：R446.62[医药卫生—诊断学]