尿液常规分析干化学法24 h内稳定性评价  

Stability of routine urine dry chemical method in 24h

作　　者：王刚强 程翔 郑善銮 WANG Gangqiang;CHENG Xiang;ZHENG Shanluan(People's Liberation Army Center for Clinical Laboratory Medicine,the First Affiliated Hospital of the Fourth Military Medical University,Xi'an 710032,Shaanxi,China)

机构地区：[1]第四军医大学第一附属医院全军临床检验医学中心,陕西西安710032

出　　处：《检验医学》2017年第9期818-821,共4页Laboratory Medicine

摘　　要：目的探讨尿液常规分析干化学法24 h内重复检测结果的稳定性。方法选取318例患者新鲜尿液标本,在收集到标本后立刻上机检测,所得结果即为0 h结果,然后分别在常温(25±2)℃和4℃下,2、4、6、24 h内检测尿液干化学10项:酸碱度(p H值)、比重(SG)、白细胞(LEU)、隐血(ERY)、亚硝酸盐(NIT)、蛋白质(PRO)、葡萄糖(Glu)、胆红素(Bil)、尿胆原(URO)、酮体(KET)。结果与0 h时相比,常温(25±2)℃下4 h LEU、ERY的变化差异有统计学意义(P<0.05),4℃下6 h LEU、ERY的变化差异有统计学意义(P<0.05);其他项目结果无论是常温还是4℃冷藏在24 h内变化差异均无统计学意义(P>0.05)。结论尿液常规分析干化学法不同项目在24 h内的稳定性不同,常温保存应于2 h内完成检测,4℃运送保存应在4 h内完成检测。对于一些特殊疾病的患者标本应尽可能优先处理,从而保证其结果稳定、准确。Objective To investigate the stability of routine urine dry chemical method in24h.Methods Atotal of318fresh urine specimens were collected.The specimens were determined immediately after collection,andthe results were named as the results of0h.The specimens were determined after collection for2,4,6and24h,under room temperature(25±2)℃and4℃,respectively.The pH value,specific gravity(SG),leukocyte(LEU),erythrocyte(ERY),nitrite(NIT),protein(PRO),glucose(Glu),bilirubin(Bil),urobilinogen(URO)and ketone body(KET)were determined.Results Compared with the results of0h,the results of LEU and ERY of4h at room temperature had statistical significance(P<0.05).The changes of LEUand ERY of6h at4℃had statistical significance(P<0.05),but there was no statistical significance for otherparameters within24h at either room temperature or4℃(P>0.05).Conclusions The stabilities of urine drychemical method for different parameters within24h are different.The urine specimens should be determined within2h at room temperature,or within4h at4℃.Some urine specimens from patients with special diseases should bedetermined as soon as possible,to ensure stability and accuracy.

关 键 词：尿液 放置时间 稳定性 

分 类 号：R446.1[医药卫生—诊断学]