福建省浙江红花油茶遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Camellia chekiangoleosa Hu in Fujian Province

作　　者：宋倩倩 付茂旺 何艺凡 林文俊[1,2] 陈辉 郑国华[1,2] 李煜 SONG Qian-qian;FU Mao-wang;HE Yi-fan;LIN Wen-jun;CHEN Hui;ZHENG Guo-hua;LI Yu(College of Forestry,Fujian Agriculture and Forestry University;Camellia Engineering Technology Research Center,Fuzhou 350002,China)

机构地区：[1]福建农林大学林学院 [2]福建省油茶工程技术研究中心,福州350002

出　　处：《四川农业大学学报》2017年第3期370-374,共5页Journal of Sichuan Agricultural University

基　　金：福建省科技重大专项(2013NZ0001-1);福建省科技厅平台建设项目(2010N2001);高等学校博士学科点专项科研基金(20123515110010)共同资助

摘　　要：【目的】为福建省浙江红花油茶的良种选育与充分开发利用提供参考。【方法】以福建省13个居群198份浙江红花油茶为实验材料,对其进行遗传多样性的ISSR分子标记分析。【结果】17个ISSR引物扩增出167条位点,其中多态性位点142条,平均多态位点百分率为85.03%。13个居群浙江红花油茶的遗传距离的变化范围为0.145 9~0.509 4。通过遗传距离进行聚类分析,可将13个天然居群的浙江红花油茶分为4个大类。【结论】福建省不同地区的浙江红花油茶遗传多样性丰富,居群间亲缘关系差异明显。【Objective】The objective of this study was to provide a reference for fully exploiting and uti原lizing the germplasm resources of Camellia chekiangoleosa Hu in Fujian province.【Method】The geneticdiversity of13populations of C.chekiangoleosa in Fujian Province was analyzed by ISSR molecularmarker technique.【Results】The results showed that17primers were obtained and167loci were ampli原fied;in addition,polymorphic loci was142and the average percentage of polymorphic loci was85.03%.The genetic distance of13populations of C.chekiangoleosa in Fujian province ranged from0.7451to0.9402.The13populations of C.chekiangoleosa in Fujian province were divided into four groups bycluster analysis based on genetic distance.【Conclusion】The genetic diversity of C.chekiangoleosa in dif原ferent areas of Fujian Province was rich and the genetic relationship among populations was significant.

关 键 词：浙江红花油茶 ISSR 遗传多样性 聚类分析 

分 类 号：S664.2[农业科学—果树学]