苯并芘诱导肝脏的炎症损伤及EGFR的表达变化  

Expression of EGFR in benzopyrene-induced hepatic inflammation

作　　者：邓燕霞 古盼[1] 刘海龙 郑凯[2] 刘建军 黄新凤[2] 李习艺[1] 黄海燕[2] DENG Yanxia;GU Pan;LIU Hailong;ZHENG Kai;LIU Jianjun;HUANG Xinfeng;LI Xiyi;HUANG Haiyan(School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi;Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention,Shenzhen 518055, Guangdong, China)

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]深圳市疾病预防控制中心现代毒理学重点实验室,广东深圳518055

出　　处：《癌变．畸变．突变》2017年第5期383-386,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81370080;81001261);深圳市未来产业专项资金(ZDSYS20140509101335476)

摘　　要：目的:探讨苯并芘(Ba P)长期暴露对大鼠肝脏的炎症损伤及表皮生长因子受体(EGFR)的表达变化。方法:选择6周龄雌性SPF级SD大鼠36只,将其随机分为染毒组和对照组,每组18只,染毒组胸腔注射10 mg/m L的玉米油-苯并芘0.2 m L,对照组注射0.2m L玉米油,每2周注射1次,共4次,连续观察1年,期间记录大鼠的生存状况和体质量变化,实验结束时收集大鼠肝脏组织,利用HE染色进行病理分析,同时采用免疫组化、免疫荧光和Western blot检测肝脏组织中EGFR蛋白的表达变化。结果:与对照组比较,染毒组实验期间大鼠体质量和生长未见明显异常;病理分析发现Ba P作用后的大鼠肝脏出现了明显的碎片状坏死和炎性浸润;免疫组化、免疫荧光结果均显示染毒组大鼠肝脏内EGFR表达增强;Western blot检测发现,染毒组EGFR蛋白表达水平(0.915±0.253)较对照组(0.452±0.095)明显升高(P<0.05)。结论:Ba P诱导肝脏炎症的同时可激活EGFR高表达,推测Ba P诱发的炎症与EGFR的表达密切相关。OBJECTIVE:To investigate the effect of long-term benzopyrene(BaP)exposure on expression ofepithelial growth factor receptor(EGFR)in SD rat liver.METHODS:36healthy SD female rats were randomly dividedinto exposure and control groups with18rats per group.Rats in the exposure groups were injected with0.2mL of10mg/mLcorn-benzopyrene through chest punctured,once per2weeks for2months.Rats in the control group were injected with0.2mL corn oil.All rats were observed the survival status and body mass changes for one year.Immunohistochemical,immunofluorescence and Western blot assays were used to detect expression of EGFR protein in livers and HE staining forpathological effects.RESULTS:HE staining showed significant fragmentation necrosis and inflammatory infiltration afterbenzopyrene exposure.Immunohistochemistry and immunofluorescence showed that expression of EGFR in the liver of theexposure group was significantly higher than that of the control group.Based on Western blot results(0.452±0.095,0.915±0.253),expression level of EGFR protein in the exposed group was significantly higher than that in the controlgroup(P<0.05).CONCLUSION:BaP induced hepatic inflammation and EGFR overexpression,suggesting that the twoeffects were closely related.

关 键 词：苯并芘 肝损伤 炎症 表皮生长因子受体 

分 类 号：R994.6[医药卫生—毒理学]