复硝酚钠与GA_3复配对美国红栌组培苗生长的影响  

Effects of combination sodium nitraphenolate+GA_3 on Cotinus coggygria tissue culture plants

作　　者：杨艳敏[1] 贾海丽[1] 潘一展[1] YANG Yan-min;JIA Hai-li;PAN Yi-zhan(Landscape Architecture College,Shangqiu University,Shangqiu,Henan 476000,China)

机构地区：[1]商丘学院风景园林学院,河南商丘476000

出　　处：《南方农业学报》2017年第9期1653-1658,共6页Journal of Southern Agriculture

基　　金：河南省重点科技攻关项目(13210211139);河南省高等学校青年骨干教师项目(2016GGJS-180);商丘市重点科技攻关项目(153021);商丘学院科研创新团队项目(170517)

摘　　要：【目的】探讨生长调节剂复硝酚钠与赤霉素(GA_3)复配对美国红栌组培苗生长的影响,为缩短美国红栌组培苗培养周期提供技术支持。【方法】在B5培养基中分别添加不同浓度的复硝酚钠+GA_3复配液培养美国红栌组培苗,25 d后统计各处理组培苗的单株叶片数、株高、鲜重、干重、生根率及根系条数,分析复硝酚钠+GA_3复配对美国红栌组培苗生长的影响。【结果】0.020 mg/L复硝酚钠+30.0 mg/L GA_3复配液处理的美国红栌组培苗生长效果最佳,单株叶片数、株高、鲜重和干重均显著高于对照(CK)及其他浓度处理(P<0.05),其中,分别比CK提高83.3%、71.8%、86.6%和85.8%,生根效果也较优,生根率最高,达95.2%,且根系条数较多,叶片肥厚、健壮,植株长势良好。相关性分析结果表明,复硝酚钠+GA_3复配液浓度与美国红栌组培苗的单株叶片数、株高、鲜重、干重、根系条数和生根率呈极显著正相关(P<0.01)。【结论】在B5培养基中添加0.020 mg/L复硝酚钠+30.0 mg/L GA_3复配液,可促进美国红栌组培苗生长,进而缩短其培养周期,加快种苗繁育速度。【Objective】Effects of plant growth regulator combination sodium nitraphenolate+gibberellin(GA3)at different concentrations on the growth of Cotinus coggygria were explored in order to provide technical support for shortening the culture cycle of tissue culture plants.【Method】B5medie adding sodium nitraphenolate+GA3of different combinations were used to culture C.coggygria.Leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number were investigated after25d of culture to study the effects of adding sodium nitraphenolate+GA3on the tissue culture plants.【Result】0.020mg/L sodium nitraphenolate+30.0mg/L GA3had the optimal effects on C.coggygria tissue culture plants.Leaf number per plant,plant height,fresh weight,dry weight were significantly higher than those in control(CK)and other treatments(P<0.05).Leaf number per plant,plant height,fresh weight,dry weight increased83.3%、71.8%、86.6%and85.8%respectively compared with CK.Rooting was also better than CK and the highest rooting rate reached95.2%.The plants were strong with large root number,thick leaves and sound growth state.Correlation analysis indicated that there was extremely positive correlation between nitraphenolate+GA3concentrations and leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number(P<0.01).【Conclusion】Adding0.020mg/L sodium nitraphenolate+30.0mg/L GA3into B5medium can improve the growth for C.coggygria tissue culture plants,and shorten the culture cycle and speed up the breeding process.

关 键 词：美国红栌 组培苗 复硝酚钠 赤霉素(GA3) 复配 

分 类 号：S685.14[农业科学—观赏园艺]