丹酚酸A对大鼠脑缺血再灌注后细胞凋亡的保护作用及其机制  

The protective effect of salvianolic acid A against apoptosis in middle cerebral artery ischemia reperfusion model and its mechanism

作　　者：彭潇[1] 方世记[2] 郑丽云[1] 邱伟文 PENG Xiao;Fang Shiji;Zheng Liyun;Qiu Weiwen(Department of Neurology the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000;Department of Intervention the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000;Department of Neurology, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000)

机构地区：[1]温州医科大学附属第五医院神经内科,浙江丽水323000 [2]温州医科大学附属第五医院介入科,浙江丽水323000 [3]温州医科大学附属第六医院神经内科,浙江丽水323000

出　　处：《温州医科大学学报》2017年第9期674-677,共4页Journal of Wenzhou Medical University

基　　金：浙江省医药卫生科技计划项目(2017KY170);丽水市公益性技术应用项目(2016GYX39;2016GYX25)

摘　　要：目的:探讨丹酚酸A(Sal A)对大鼠脑缺血再灌注后细胞凋亡的保护作用及其机制。方法:采用线拴法构建大脑中动脉缺血再灌注(MCA-IR)模型。将正常SD大鼠随机分为假手术组(对照组)、MCA-IR模型组(MCA-IR组)、Sal A+MCA-IR组(Sal A组);Sal A组于建模前1周每日腹腔注射1.0、2.5、5.0mg/kgSal A。于再灌注后24h取梗死脑组织用TUNEL法检测脑细胞凋亡,Westernblot检测磷酸化Akt(p-Akt)和Akt的表达,免疫组织化学法检测海马区胞浆型磷脂酶A2(cPLA2)的表达。结果:Sal A组海马CA1区细胞凋亡率和MDA含量较MCA-IR组降低,而SOD量较MCA-IR组显著增加(P<0.05),MCA-IR组海马区p-Akt表达较对照组显著下降(P<0.05),而cPLA2较对照组显著增高(P<0.05),1.0、2.5、5.0mg/kgSal A预处理后,MCA-IR模型大鼠海马区cPLA2表达显著降低(P<0.05),而p-Akt表达显著增加(P<0.05)。结论:Sal A降低缺血再灌注损伤后细胞凋亡,起到损伤保护作用,其机制与其降低cPLA2表达,激活Akt信号通路有关。Objective:To investigate the protective effect of salvianolic acid A(Sal A)against apoptosisin cerebral ischemia-reperfusion model and its mechanism.Methods:SD rats were randomly divided into shamoperation group(sham group),middle cerebral artery ischemia reperfusion model(MCA-IR)group(MCA-IRgroup),Sal A pretreatment and MCA-IR group(Sal A+MCA-IR group).MCA-IR was established in MCA-IRgroup and Sal A+MCA-IR group in which rats were pre-treated with1.0,2.5,5.0mg/kg Sal A via intraperitonealinjection daily for1week.The artery were reperfused for24h and rats were sacrificed.TUNEL was used to assayedthe cell apoptosis,immunohistochemistry was implied to test the expression of cPLA2and Western blotwas undergone to analyzed p-Akt and total Akt.Immunohistochemistry was used to analyze the expression of cPLA2.Results:The apoptosis rate and MDA level in Sal A+MCA-IR group was lower,but SOD was higher thanthat in MCA-IR group(P<0.05).The expression of p-Akt in Hippocampus of MCA-IR model decreased,whilethe expression of cPLA2increased significantly compared with control group.1.0,2.5,5.0mg/kg Sal A pretreatmentreversed the decrease of p-Akt and increase of cPLA2.Conclusion:Sal A inhibits the apoptosis throughdecreasing the expression of cPLA2and activating Akt pathway signaling in MCA-IR model.

关 键 词：丹酚酸A 细胞凋亡 蛋白激酶B 脑缺血再灌注 大鼠 

分 类 号：R285[医药卫生—中药学] R743.1[医药卫生—中医学]