絮凝剂对紫云英根瘤菌发酵液絮凝效果的研究  

Flocculating Efficiency of Rhizobium astragalus with Flocculants

作　　者：吴湘龙 雷潇 易世鸣 刘欢 孟祥池 刘宇波 曾奥 WU Xiang-long;LEI Xiao;YI Shi-ming;LIU Huan;MENG Xiang-chi;LIU Yu-bo;ZENG Ao(Hunan Institute of Microbiology, Changsha 410009, PRC)

机构地区：[1]湖南省微生物研究院,湖南长沙410009

出　　处：《湖南农业科学》2017年第9期10-12,共3页Hunan Agricultural Sciences

基　　金：国家自然科学基金(31501698)

摘　　要：根瘤菌发酵会产生大量的胞外粘液,使发酵液变得十分粘稠,从而严重影响发酵液的固液分离。为降低发酵液的菌体收集难度,研究在优化根瘤菌发酵水平的基础上,选用7种絮凝剂对发酵液进行处理,以絮凝率、蛋白去除率、多糖去除率、上清液菌体含量为指标进行筛选。结果表明:聚合氯化铝(50 mg/L)絮凝速率最快,蛋白多糖去除率高,但粘壁现象严重,上清菌体含量少;而阳离子聚丙烯酰胺(100 mg/L)表现出良好的上清菌体含量以及多糖去除率,且粘壁现象少,最适用于紫云英根瘤菌发酵液多糖去除和菌体的收集。Rhizobium fermentation produces a large amount of extracellular mucus,so the solid-liquid separation of mucus becomesvery difficult.In order to reduce the difficulty in bacteria collection,7flocculants were used to treat fermentation broth at an optimizedfermentation level and evaluate the flocculation effect through flocculation rate,protein removal rate,polysaccharide removal rate andsupernatant microorganisms content in this study.The results showed that aluminium polychlorid had the highest flocculation rate,highprotein removal rate and polysaccharide removal rate,but a low supernatant microorganisms content with the serious phenomenon ofadhering to wall;and cationic polyacrylamide performed a good effect of supernatant microorganisms content and polysaccharide removalrate,with a little wall-adhering phenomenon.In summary,the cationic polyacrylamide was the most suitable flocculant to Rhizobiumastragalus for the solid-liquid separation of mucus.

关 键 词：絮凝剂 紫云英根瘤菌 多糖 絮凝 

分 类 号：Q939.114[生物学—微生物学]